THE IFS-SPN INTERFACE

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 圣路易斯华盛顿大学的Caparon和Hultgren团队最近发现了化脓性链球菌分泌的蛋白质毒素(SPN，~36 kDa)。化脓性链球菌是一种革兰氏阳性细菌，可导致链球菌咽喉和食肉疾病。最近在与SPN相同的密码子上发现了一种新的内源性抑制因子，命名为SPN免疫因子(IfS，~22 kDa)。IFs与SPN以1：1的摩尔比形成稳定的络合物，并通过竞争性抑制作用抑制SPN的活性。在圣路易斯华盛顿大学的埃伦伯格小组中确定了IF的晶体结构(未发表的数据)。我们的目标是使用高分辨率的PLIMSTEX协议来定位IFS与SPN的绑定接口。该复合体的晶体结构正在研究中。 我们计划在来年出版这部作品。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The teams of Caparon and Hultgren at Washington University in St. Louis recently identified the protein toxin (SPN, ~36 kDa) secreted by Streptococcus pyogenes, a Gram-positive bacteria responsible for causing strep throat and flesh eating disease. A novel endogenous inhibitor, named Immunity Factor for SPN (IFS, ~ 22kDa) was recently discovered on the same codon as SPN. IFS forms a stable complex with SPN at a 1:1 molar ratio and inhibits SPN's activity by acting as a competitive inhibitor. The crystal structure of IFS was determined in the Ellenberger group at Washington University in St. Louis (unpublished data). Our goal is to use the high resolution PLIMSTEX protocol to locate the binding interface of IFS to SPN. The crystal structure of the complex is underway. We are planning to publish this work in the coming year.