P30 - Core Grant for Vision Research

描述(由申请者提供)：申请继续支持埃默里大学和邻近的亚特兰大机构的NEI视觉研究核心助学金。核心中心奖助金续期申请包括三个模块：(1)结构生物学和成像，(2)功能基因组学和蛋白质组学，以及(3)生物信息学和生物统计学。过去的核心拨款支持42名教师(包括由31名NEI资助的首席研究员颁发的36个单独奖项)、16名博士后研究员、6名博士后研究员之间的协作愿景研究和服务，并在过去5年中发表了500多篇同行评议的出版物。结构生物学和成像模块在此次更新中增加了对免疫组织化学、共聚焦显微镜、荧光图像分析以及光学和电子显微镜服务的现有支持。功能基因组学和蛋白质组学模块为啮齿动物的视觉表型鉴定增加了新的功能，包括SD-OCT、扫描激光眼底镜、部分相干干涉测量、高精度显微测量、光折射、房角镜、反弹眼压测量、ERG和OKT，以满足现有的 寡核苷酸合成、高效液相分析、质谱学、聚合酶链式反应、激光捕获显微切割、基因和蛋白质测序以及微阵列分析。生物信息学和生物统计模块提供生物统计学、生物信息学、数据管理、电子笔记本、数据备份和高级计算分析。核心由计划主任和每个模块的一至两名模块主任管理；核心赠款咨询委员会将协助模块主任确保充分和公平地使用设施，并根据需要就创建新资源或消除未充分利用的组件提供建议。核心模块已经并将继续通过提供共同的集中服务来提高研究效率，以鼓励和促进教职员工之间的合作研究，并吸引其他学术学科参与愿景研究。

DESCRIPTION (provided by applicant): Continued support is requested forth NEI Core Grant for Vision Research at Emory University and neighboring Atlanta institutions. This Core Center Grant renewal application includes three Modules: (1) Structural Biology and Imaging, (2) Functional Genomics and Proteomics, and (3) Bioinformatics and Biostatistics. The past core grant supported collaborative vision research and service among 42 faculty (including 36 separate awards by 31 NEI funded principal investigators),16 postdoctoral fellows, 6 predoctoral fellows and generated 500+ peer-reviewed publications in the past 5 years. The Structural Biology and Imaging Module adds ultrasonography in this renewal to existing support for immunohistochemistry, confocal microscopy, fluorescence image analysis, and light and electron microscopy services. The Functional Genomics and Proteomics module adds new capabilities for visual phenotyping of rodents, including SD-OCT, scanning laser ophthalmoscopy, partial coherence interferometry, high precision micrometry, photorefraction, gonioscopy, rebound tonometry, ERG, and OKT, to pre-existing services for oligonucleotide synthesis, HPLC analyses, mass spectrometry, PCR, laser capture microdissection, gene and protein sequencing, and microarray analysis. The Bioinformatics and Biostatistics Module provides biostatistics, bioinformatics, data management, electronic notebooks, data backups, and advanced computational analyses. The Core is administered by the Program Director and one-two Module Directors for each of the Modules; a Core Grant Advisory Committee will aid the module directors to insure full and equitable use of the facilities and to advise on creation of new resources based on need or eliminate components that are underutilized. The Core Modules have and will continue to increase research productivity by providing common centralized services to stimulate and facilitate collaborative studies among faculty and to attract other academic disciplines to vision research.