IMAGING CORE

核心A（成像）对于子宫内胎儿和畸胎瘤的MRI成像至关重要，并最终用于跟踪 干细胞移植体外实时和激光扫描共聚焦，定量FRAP，宽场和电子显微镜 显微镜也提供给所有三个子项目，以评估多能性活细胞指标的动态， hESC、nhpESC和PG细胞，以便更好地定义多能状态的特征。成像核心提供 在体外、子宫内和体内对干细胞动力学进行异常高灵敏度的检测，这对每一种疾病都至关重要。 研究项目。为了确保Imaging Core的最大效用，我们将联合收割机结合体内非侵入性 常规、共聚焦和电子显微镜体外成像MRI。在宏观整体上 在动物水平上，我们将利用新的小动物成像技术，如MRI。成像的主要作用 核心将是：对妊娠猴进行纵向MRI研究，并监测存活率和表型 嵌合体胎儿和胎盘在整个妊娠期间的差异;采用高分辨率3D MRI对固定和活体 ES衍生的NHP胚胎，以检查干细胞的贡献;并监测体内畸胎瘤的形成。 目标1.成像核心将为所有三个项目提供全身非侵入性功能成像。核心是 负责跟踪ES和EG嵌合胚胎中的细胞谱系贡献以及移植hES的命运 细胞该核心还将使用高分辨率MRI来绘制胎儿发育和畸胎瘤形成，并执行 在切片之前对切除的畸胎瘤进行磁共振显微镜检查。该核心还将致力于发展 以及优化用于跟踪体内干细胞的技术，包括用于MRI的ES和EG细胞的SPIO标记。 目标2.成像核心将为固定、免疫细胞化学、活体 细胞共聚焦，实时动态和电子显微镜，用于测定多能和 分化的人胚胎干细胞，特别是活的和固定的多能标记物测定，以更好地表征 未分化的人类和非人类灵长类动物ES和EG细胞。该核心还将进行ES的基础研究 和EG细胞对嵌合胚胎的贡献以及ES和EG细胞的印迹状态，以及 畸胎瘤和胚状体的免疫细胞化学和免疫组织化学。

Core A (Imaging) is vital for the MRI imaging of fetuses in utero and teratomas, and ultimately for tracking transplanted stem cells. In vitro real-time and laser-scanning confocal, quantitative FRAP, wide-field and electron microscopy are also provided to all three subprojects to evaluate the dynamics of live cell indicators of pluripotency in hESC, nhpESC and PG cells in order to better define characteristics of the pluripotent state. The imaging core provides exceptional high-sensitivity detection of stem cell dynamics in vitro, in utero and in vivo, essential for each of the research projects. To ensure maximum utility of the Imaging Core, we will combine the strengths of in vivo noninvasive MRI with in vitro imaging by conventional, confocal, and electron microscopy. At the macroscopic whole animal level, we will utilize new small animal imaging technologies, such as MRI. The primary roles of the Imaging Core will be to: perform longitudinal MRI studies of pregnant monkeys and monitor viability and phenotypic differences of the chimeric fetus and placenta throughout pregnancy; employ high-resolution 3D MRI of fixed and live ES-derived NHP embryos to examine stem cell contributions; and monitor teratoma formation in vivo. Aim 1. The imaging core will provide total body non-invasive functional imaging to all three projects. The core is responsible for tracking cell lineage contributions in ES and EG chimeric embryos and the fates of transplanted hES cells. The core will also use high resolution MRI to map fetal development and teratoma formation, and perform magnetic resonance microscopy on excised teratomas prior to sectioning. This core will also work towards developing and optimizing techniques for the tracking of stem cells in vivo including SPIO labeling of ES and EG cells for MRI. Aim 2. The imaging core will provide instrumentation, oversight and guidance for fixed, immunocytochemistry, live cell confocal, real-time dynamic and electron microscopy for assaying cell structure and dynamics in pluripotent and differentiated human embryonic stem cells, especially live and fixed pluripotent marker assays to better characterize undifferentiated human and nonhuman primate ES and EG cells. This core will also perform fundamental studies of ES and EG cell contributions to chimeric embryos and imprinting status of ES and EG cells, as well as immunocytochemistry and immunohistochemistry of teratomas and embryoid bodies.