New Phosphated Polymers Prevent Microbial Colonization

描述（由申请人提供）：本研究项目的目标是开发新一代义齿基聚合物，可以控制微生物定植并预防义齿引起的口炎。这些新的聚合物将通过在聚甲基丙烯酸甲酯[PMMA]中加入磷酸基团来合成，以防止假牙感染。新聚合物中的磷酸基团将为唾液阳离子抗菌剂选择性吸附到义齿表面提供所需的阴离子表面。这将导致假牙表面形成防御膜层。目前可用的PMMA，由于缺乏离子表面和防御义齿膜成分，不抑制微生物定植。本研究项目涉及：1)含不同数量磷酸基和非磷酸PMMA的磷酸PMMA的合成和聚合物的表征；2)利用组蛋白5和防御蛋白片段评价该聚合物吸附和解吸唾液抗菌剂的效果；3)测定白色念珠菌、血链球菌、口腔链球菌、戈多氏链球菌和变形链球菌吸附的磷酸高分子抗菌剂的杀微生物活性；4)描述对唾液抗菌剂具有高亲和力的聚合物的物理和机械性能，并根据ADA/ISO指南抑制微生物粘附在聚合物表面；5)评估聚合物样品对人口腔上皮细胞系、人原代牙龈成纤维细胞和标准L-929细胞系的体外细胞毒性，以确定牙科材料的安全要求。相关性：本研究将确定新的、安全的、可负担得起的磷酸PMMA聚合物，作为义齿基托或义齿基托衬里材料，用于预防和治疗义齿感染。这些新的聚合物将表现出与正常口腔表面相当的物理、机械和生物特性，并将对与现有PMMA聚合物相关的假牙引起的感染的治疗费用产生重大影响。这些新聚合物的使用可以改善大量假体使用者的口腔健康。磷酸化PMMA聚合物在临床和商业应用方面具有很大的潜力。

DESCRIPTION (provided by applicant): The goal of this research project is to develop a new generation of denture-base polymers that could control microbial colonization and prevent denture-induced stomatitis. These new polymers will be synthesized by the incorporation of phosphate groups within poly (methyl methacrylate) [PMMA] to prevent denture infection. Phosphate groups in the new polymers will provide the required anionic surface for the salivary cationic antimicrobials to be selectively adsorbed onto the denture surface. This will result in the formation of a defense pellicle layer on the denture surface. The currently available PMMA, due to the absence of ionic surface and the defense denture pellicle component, does not inhibit microbial colonization This research project involves: 1) Synthesis of phosphated PMMAs containing varied amounts of phosphate groups and non-phosphated PMMA and characterization of polymers; 2) Assessment of the efficacy of this polymer to adsorb and desorb salivary antimicrobials using histatin 5 and a defensin fragment; 3)Determination of the microbicidal activity of antimicrobials adsorbed phosphated polymers using Candida albicans, Streptococcus Sanguis, Streptococcus oralis, Streptococcus gordonii and Streptococcus mutans; 4) Delineation of the physical and mechanical properties of polymers that exhibit high affinity for salivary antimicrobials, and inhibit microbial adhesion to the polymer surface following the ADA/ISO guidelines; 5) Assessment of the in vitro cytotoxicity of polymer specimens against a human oral epithelial cell line, human primary gingival fibroblast cells, and the standard L-929 cell line to ascertain the safety requirements for Dental materials. Relevance: This proposed research will identify new, safe and affordable phosphated PMMA polymers that could serve as denture-base or denture base lining materials for the prevention and treatment of denture infection. These new polymers will exhibit physical, mechanical and biological properties comparable to those of normal oral surfaces, and will have significant impact on the expenses involved in the treatment of denture-induced infection associated with the existing PMMA polymer. The use of these new polymers could improve the oral health of a significant population of prosthesis users. The phosphated PMMA polymers could have great potential for clinical and commercial applications.