ShEEP Request for Chromium System

10 x Genomics Chromium系统是我们研究的重要工具。10倍 Genomics Chromium系统由Chromium控制器Chromium i7 Multiplex组成 试剂盒、铬单细胞3'文库和凝胶珠试剂盒以及铬单细胞A芯片试剂盒。 这些组件一起用于进行基因组“连接读段”文库制备， 测序这些组件只能与Chromium控制器一起使用，不能使用 独立地。有许多研究人员正在研究特定的单细胞类型， 阐明各种生物过程的作用机制。这在原代细胞中很难做到 因为有许多细胞类型必须彼此分离。该系统可以识别 单个细胞，因此可以研究不同的细胞群体，而不会有其他细胞类型混淆。 结果这款单细胞和DNA分析10 X Genomics™由10 x GemCode™组成， 为Chromium系统提供创新的试剂输送， 信息在一个巨大的速度和规模。Chromium控制器可以快速封装 将单个细胞或基因组DNA分成100，000至1，000，000个可唯一寻址的分区， 分钟，每个包含用于下游基因组分析的识别条形码， Chromium软件套件。它将使我们能够探索衰老和其他疾病是如何 影响主要类型的结构变异（SV），如缺失、倒位和易位，以及 还允许对短读测序仪无法获得的基因进行测序。10 X Genomics™将 还允许跨> 10 Mb单倍型区块分析相位SNV、插入缺失和SV。最后 将允许从少至1 ng的基因组DNA产生高质量的文库。 这项技术对VA研究非常重要。有14名主要调查员 在VAPAHCS谁将应用这项技术在他们的研究，以分析基因表达在一个 单细胞水平，以阐明衰老、类固醇生成、伤口/骨愈合、脂质 代谢，细胞免疫反应，T细胞活化，骨疾病，肥胖，海湾战争 疾病，以及许多其他代谢疾病和条件，特别是影响 退伍军人人口。从这些项目中获得的知识将增强我们的理解 与各种疾病的病理状况和发病机制有关的分子事件 影响退伍军人健康的因素。

The 10x Genomics Chromium System is an important instrument for our research. The 10X Genomics Chromium System is comprised of the Chromium Controller, Chromium i7 Multiplex kit, Chromium Single Cell 3' Library and Gel Bead Kit, and the Chromium Single Cell A Chip kit. These components are used together to perform Genome “linked read” library preps and sequencing. These components will only work with the Chromium Controller and cannot be used independently. There are many investigators that are researching specific single cell types to elucidate mechanisms of action of various biological processes. This is hard to do in primary cells as there are many cell types that must be isolated from one another. This system can identify single cells, so different cell populations can be studied without other cell types confounding the results. This Single Cell and DNA Analysis 10X Genomics™ consists of 10x GemCode™ that fuels Chromium System with an innovative reagent delivery of previously inaccessible genomic information at a massive rate and scale. The Chromium Controller can quickly encapsulate individual cells or genomic DNA into 100,000s to 1,000,000s of uniquely addressable partitions in minutes, each containing an identifying barcode for downstream genomic analysis with the Chromium Software Suite. It will allow us to explore how aging and other diseases conditions impact major classes of structural variants (SVs) like deletions, inversions and translocations, and also allows the sequencing of genes inaccessible to short-read sequencers. 10X Genomics™ will also allow analysis of Phase SNVs, indels and SVs across > 10 Mb haplotype blocks. Finally, it will allow generation of high-quality libraries from as little as 1 ng of genomic DNA. This technology is extremely important to VA research. There are fourteen principle investigators at VAPAHCS who will apply this technology in their research to analyze gene expression at a single cell level to elucidate mechanisms of aging, steroidogenesis, wound/bone healing, lipid metabolism, cellular immune responses, T cell activation, bone diseases, obesity, Gulf War Illness, as well as many other metabolic diseases and conditions that particularly affect the Veterans population. The knowledge gained from these projects will enhance our understanding of the molecular events connected with the pathological conditions and pathogenesis of various conditions that affect veterans'' health.