ShEEP Request for CTL ImmunoSpot S6 Universal Analyzer

我们正在申请资金购买CTL ImmunoSpot® S6通用分析仪。本文书 允许研究人员主要进行多色ELISPOT分析，沿着其他细胞， 基于应用程序。目前，无论是在俄克拉荷马州城市退伍军人医疗研究机构 中心（OKC VAMC）也没有附属的俄克拉荷马州健康科学中心大学 这台高端ELISPOT分析仪众所周知，免疫细胞在许多疾病中起着关键作用。 包括癌症在内的疾病。现在越来越明显的是，免疫细胞异质性的变化 潜在地对疾病进展产生积极或消极的影响。但过去 只有有限的技术才能让研究人员研究免疫细胞的异质性，免疫细胞， 细胞与其他细胞类型的相互作用及其在组织微环境中的分子影响 更重要的是，免疫标记物在组织微环境中和系统地相互作用。 免疫监测是复杂的，更好的理解需要多路复用。CTL ImmunoSpot® S6通用分析仪可进行MTT和B细胞荧光斑点分析， 活/死PBMC计数，以及更多。这是一个有吸引力的替代流式细胞术 分析. CTL ImmunoSpot® S6通用分析仪还允许研究者进行其他 应用在微观（单细胞和亚细胞颗粒）以及宏观 水平（菌落和噬菌斑）。CTL ImmunoSpot® S6 Universal的主要应用领域 分析仪已进行多色ELISPOT分析。CTL专门采用了一种技术 旨在避免激发交叉污染，能够准确检测多达八个 分析物共表达细胞，包括多功能T细胞。此外，基于图像分析的 使用CTL ImmunoSpot® S6通用分析仪进行细胞的多参数分析是有用的 粘附或非粘附细胞都一样，并且用于分析的样品大小可以低至 一个细胞。 一项对OKC VAMC所有VA研究人员的调查显示，超过80%的研究人员可能 有兴趣使用CTL ImmunoSpot® S6通用分析仪研究抗原的调节- 特异性T细胞诱导和活化。该仪器将用于六个主要的 谁拥有VA优异奖赠款资助或待定的用户。我们还预计，研究人员在其他 VA中心将使用该仪器。

We are requesting funds to purchase a CTL ImmunoSpot® S6 Universal Analyzer. This instrument allows investigators primarily to perform multi-color ELISPOT analysis along with other cell- based applications. Currently, neither the research facility at the Oklahoma City VA Medical Center (OKC VAMC) nor the affiliated University of Oklahoma Health Sciences Center has this high-end ELISPOT analyzer. It is well known that immune cells play a critical role in many diseases including cancer. It is now becoming evident that changes in immune cell heterogeneity potentially impacts either positively or negatively on the disease progression. However, in the past only limited technology allowed investigators to study heterogeneity of immune cells, immune cells interaction with other cell types and their molecular impact in the tissue microenvironment and more so, immunological markers interface in tissue microenvironment and systemically. Immune monitoring is complex and better understanding requires multiplexing. CTL ImmunoSpot® S6 Universal Analyzer permit multicolor T and B cell fluorospot analysis, live/dead PBMC counting, and much more. This is an attractive alternative to flow cytometry analysis. CTL ImmunoSpot® S6 Universal Analyzer also allows investigators for other applications at the microscopic (single cells and subcellular particles) as well as the macroscopic level (colonies and Plaques). The primary field of application for CTL ImmunoSpot® S6 Universal Analyzer has been multi-color ELISPOT analysis. CTL employs a technology specifically designed to avoid excitation cross-contamination, enabling the accurate detection of up to eight analyte co-expressing cells, including polyfunctional T cells. In addition, image analysis-based multi-parameter analysis of cells with CTL ImmunoSpot® S6 Universal Analyzer can be useful with both adherent or non-adherent cells alike, and the sample size for analysis can be as low as one cell. A survey of all VA researchers at OKC VAMC showed over 80% of the researchers are potentially interested to use CTL ImmunoSpot® S6 Universal Analyzer to study the modulation of antigen- specific T cell induction and activation in their research. This instrument will be used by six major users who have VA Merit grants funded or pending. We also anticipate that researchers at other VA centers will use this instrument.