ShEEP Request for Laser Scanning Confocal Microscopy Acquisition and Analysis System

该建议是一个共享的激光扫描共聚焦显微镜采集和分析系统， 主要用户组（六名优异奖资助的PI）以及VA安阿伯健康中心的其他VA研究人员 Care System（VAAAHS）在活体和保存组织中进行高分辨率分析。研究 重点关注优先影响退伍军人群体的疾病的细胞发病机制。研究包括 火器引发的听力和平衡丧失、部署相关慢性肺疾病、PTSD、COPD、CNS 感染和神经退行性疾病的子集，某些类型的癌症（肿瘤免疫疗法）。这些 疾病的特征是特定的，往往是微妙的，微观解剖组织的变化和详细的 分子水平的鉴定和评估对于执行研究、提出新的治疗方案至关重要。 机会，并随后测试其有效性。这一需要没有得到充分满足。请求的 系统由Leica SP-8倒置激光扫描共聚焦显微镜和Tokia HIT载物台组成 用于活细胞成像孵育系统。它将有多个激光器和高性能探测器， 通道、快速采集能力和高达120 nm的分辨率。空气、水、油和甘油浸泡 具有不同工作距离和数值孔径的物镜将满足我们不同用户的需求 他们的各种标本和评估。采集和图像分析软件 工作站将提供增强的成像、可视化、共同定位和旋转，以及二维和三维 D分析和测量。FRET和FRAP "向导"将允许这些应用程序。引入新 能力将通过提供新数据和改进数据收集和分析， 以及提高效率和吞吐量。它将把现有的研究扩展到新的方向， 识别和评估，否则是不可能的，例如，通过允许新的多个 共同标记分子和深入样本的细胞成分，或允许实时动态活细胞 成像、FRET和弗拉姆分析。它还将创造新的能力，帮助我们未来的赠款申请 教师和新的学习机会，学员在VAAAHS网站。所要求的系统将加强 并将VAAAHS对影响我们退伍军人群体的疾病的研究扩展到开发治疗药物， 最终让我们的患者受益。

This proposal is for a shared Laser Scanning Confocal Microscopy Acquisition and Analysis System to allow a Major User group (six Merit Award funded PIs) as well as other VA researchers at the VA Ann Arbor Health Care System (VAAAHS) to conduct high-resolution analysis in both living and preserved tissues. Research focuses on cellular pathogenesis of diseases that preferentially affect veteran populations. Studies include firearm triggered loss of hearing and balance, Deployment-Related Chronic Lung Disease, PTSD, COPD, CNS infections and subset of neurodegenerative disorders, certain types of cancer (tumor immunotherapy). These diseases are characterized by specific, and often subtle, micro-anatomic tissue changes and a detailed identification and assessment at the molecular level is critical to execute studies, propose new treatment opportunities and subsequently test their effectiveness. This need is not being adequately met. The requested system is composed of Leica SP-8 inverted laser scanning confocal microscope with a Tokia HIT stage-top incubation system for live cell imaging. It will have multiple lasers and high capability detectors with adjustable channels, rapid acquisition capacity and up to 120 nm resolution. Air, water, oil and glycerol immersion objectives with varying working distances and numerical apertures will match the needs of our diverse user group for their variety of specimens and assessments. Software on both acquisition and image analysis workstations will provide for enhanced imaging, visualization, co-localization and rotation as well as 2-D and 3- D analysis and measurement. FRET and FRAP “wizards” will allow these applications. Introducing new capabilities will enhance current studies by providing new data and improved data collection and analyses as well as increasing efficiency and throughput. It will extend current studies into new directions by allowing identifications and assessments that would not otherwise be possible, for example, by allowing new multiple co-labeling of molecules and cellular elements deep into specimens or by allowing real-time dynamic live cell imaging, FRET and FRAM analyses. It will also create new capabilities aiding future grant applications of our faculty and new learning opportunities for trainees at the VAAAHS site. The requested system will enhance and extend VAAAHS research on disorders effecting our veteran population towards developing therapeutic interventions to ultimately benefit our patients.