Vitamin D Deficiency Leads to Increased Intra‐Prostatic Androgens in African American Men

项目摘要 该提案是关于促进健康相关研究多样性的研究补充材料， R21 CA231610。我提议支持实验室的一位三年级研究生，杰森·加西亚先生， 参与这个项目加西亚先生是墨西哥裔美国人，他的职业目标是“获得一个教师职位 在一所大学的职位，为寻求在大学接受教育的代表性不足的学生提供服务。 STEM领域，同时继续调查癌症差异。他设计了一种 以实现R21的目标。 目的S1：定量巨蛋白介导的血清球蛋白输入，维生素D结合 蛋白质和性激素结合球蛋白，进入前列腺细胞和组织。 这些实验使用离体组织切片和前列腺组织切片的尖端方法。 类器官为Jason提供与癌症相关的患者衍生临床前模型的专业知识 差距研究。此外，通过蛋白质操作获得的技术技能， 共聚焦显微镜和信息学定量将扩大他的专业知识的广度， 实验室设置。 培训：完成加西亚先生的目标，为他成为一名有竞争力的博士后做好准备 职位，他的最后2年培训将集中在癌症差异研究的卓越， 本科生或高中生的辅导，以及概述的几个教学要素 在提案中。

PROJECT SUMMARY This proposal is for a Research Supplements to Promote Diversity in Health-Related Research to R21 CA231610. I propose to support a third year graduate student in the lab, Mr. Jason Garcia, to work on this project. Mr. Garcia is Mexican American whose career goal is “to earn a faculty position at a university catering to underrepresented students seeking an education in the STEM fields while continuing to investigate cancer disparities.” He has designed a supplement aim to complement the Aims of the R21. Aim S1: To quantify megalin-mediated import of serum globulins, vitamin D binding protein and sex-hormone binding globulin, into prostate cells and tissues. These experiments use the cutting edge methods of ex vivo tissue slices and prostate organoids to provide Jason expertise in patient-derived preclinical models relevant to cancer disparities research. Additionally, the technical skills gained with the protein manipulation, confocal microscopy and informatics quantitation will broaden his breadth of expertise in the laboratory setting. Training: To accomplish Mr. Garcia's goal and prepare him for a competitive postdoctoral position, the last 2 years of his training will focus on excellence in cancer disparities research, mentoring of undergraduate or high school students, as well as several didactic elements outlined in the proposal.