Histology/lmage Analysis

CORE D -组织学和图像分析核心- Theresa Connors - PI 组织学和图像分析核心的目标是识别和量化的解剖基质， 在电生理学和行为核心以及各个项目中测试的功能结果。 这个核心已经发展了20多年，以确保大量标本的标准化吞吐量 由PPG的各个项目产生。我们发现，每个项目的培训人员和 提供一个组织良好和储备充足的设施，在那里工作可以在核心工作人员的指导下进行， 最有效地利用资源。组织学和图像分析核心维护一个数据库， PPG人员使用的所有组织处理、组织学和免疫组织化学（IHC）程序。在 此外，核心人员负责监督图像采集和分析系统的培训和维护。 核心主任和技术人员熟练使用各种抗体和程序 鉴定神经递质和神经调质、细胞骨架成分、细胞外基质和 粘附分子、生长因子和非神经元细胞以及荧光和DAB标记 技术.核心人员也精通图像分析的操作和应用 显微镜和软体。核心努力保持对材料和程序的质量控制， 通过：要求在使用核心资源之前进行培训， 缓冲液和溶液，定期更新抗体和程序数据库，并提供指导 核心实验室项目的规划和执行。新技术的测试和实施是一个 核心功能。这提高了PPG组织学和图像分析核心的效率， 减少新程序的启动时间，同时减少重复和浪费。具有集中 组织学和图像分析中心拥有培训、设备、用品和方法数据库， 作为计划项目中其他核心实验室的模型发展

CORE D - Histology and Image Analysis Core - Theresa Connors - PI The goal of the Histology and Image Analysis Core is to identify and quantify the anatomical substrates of functional outcomes tested in the Electrophysiological and Behavior Cores and in the individual projects. This core has evolved over 20+ years to insure standardized throughput of the large quantity of specimens generated by individual projects of the PPG. We have found that training staff from each project and providing a well organized and stocked facility where work can be performed with guidance from core staff is the most efficient way to utilize resources. The Histology and Image Analysis Core maintains a database of all tissue processing, histological and immunohistochemical (IHC) procedures used by PPG personnel. In addition, the core staff oversees training on and maintenance of our image acquisition and analysis systems. The core director and technician are skilled in working with a wide variety of antibodies and procedures used to identify neurotransmitters and neuromodulators, cytoskeleton components, extracellular matrix and adhesion molecules, growth factors, and non-neuronal cells as well as fiuorescence and DAB labeling techniques. Core personnel are also well versed in the operation and application of the image analysis microscopes and softiA/are. The core strives to maintain quality control of materials and procedures used in all PPG projects by: requiring training prior to using core resources, providing stocks of all routinely used buffers and solutions, regularly updating the database of antibodies and procedures and providing guidance in the planning and execution of projects in the core lab. Testing and implementation of new techniques is an essential Core function. This has increased the efficiency of the PPG Histology and Image Analysis Core, reducing start-up time for new procedures while also reducing duplication and waste. With centralized training, equipment, supplies and a database for methods, the Histology and Image Analysis Core has evolved as the model for other Core Labs in the Program Proiect