Core C: Biochemical and Molecular Core

项目摘要-核心C 生化和分子生物学核心(核心C)的主要目的是集中--两者都比喻为 以及对大多数单个项目通用的技术的物理支持 计划项目拨款申请。因此，这一核心是调查这一事件的重要资源 应用程序，个人在他们的实验室中，是本计划项目的重要组成部分。这个核心将 为项目调查人员提供特定关键设备的技术支持、教学和维护 为常用的分子、生化和高效液相技术集中供应来源。我们还有 开发了通过PPG网站以电子方式保存的方法/协议存储库，这些协议是 适用于该计划的所有参与者。最后，我们继续开发新的方法，使我们的 调查人员。这个核心有两(2)个不同的科学焦点。 中心一：第一个中心是支持基础分子分析，包括实时聚合酶链式反应，蛋白质分析， 免疫沉淀和免疫组织化学和细胞化学。该中心的一个具体焦点是脂肪的分级 转化为脂肪细胞和间质血管部分(SVF)。这个第一个核心中心将负责提供 对执行所有这些分析的技术帮助、对使用中心设备的支持和一般 补给。核心D直接连接到核心C，因为它为评估 核心C中产生的数据。 中心II：第二个中心是支持使用高压液体进行生化分析 ROS的高效液相色谱分析和生化测定。高效液相色谱是这一核心的真正优势，使 调查人员测量对PPG重要的各种物质(例如，NE和代谢物)。

Project Summary – Core C The primary purpose of the Biochemical and Molecular Biology Core (Core C) is to centralize—both figuratively and physically- support for techniques that are common to a majority of the individual projects with this Program Project Grant application. This core is thus an important resource for the investigators of this application, the individuals in their laboratories and is an essential part of this Program Project. This core will provide project investigators with technical support, teaching, upkeep of particular key pieces of equipment and centralize supply sources for commonly-used molecular, biochemical and HPLC techniques. We have also developed a repository of methods/protocols kept electronically through the PPG website, protocols that are available to all participants in the program. Finally, we continue to develop new methods that benefit our investigators. There are two (2) distinct scientific foci of this core. Center I: The first center is support for basic molecular analyses, including real-time PCR, Western analyses, immunoprecipitation and immunohisto- and cytochemistry. A specific focus of this Center is fractionation of fat into adipose cells and stromal vascular fractions (SVF). This first core center will be responsible for providing technical help for performing all of these assays, support for use of central pieces of equipment and general supplies. Core D connects directly to Core C in that it provides the microscopic expertise for evaluation of the data produced within Core C. Center II: The second center is support for biochemical-based analyses using high pressure liquid chromatography (HPLC) and biochemical measurement of ROS. HPLC is a real strength of this core, enabling investigators to measure a variety of substances important to the PPG (e.g. NE and metabolites).