Integrated Phosphor and Fluorescence Imager (Typhoon FLA9500)

 描述(由申请人提供)：本提案寻求资金购买台风FLA9500生物分子成像仪(GE Healthcare Life Sciences)，以取代现已过时的富士FLA3000G，后者已满足我们的共同需求15年。台风FLA9500是一种高分辨率、高灵敏度的激光扫描仪器，应用于磷成像和荧光、化学荧光和比色成像。目前的富士FLA3000G是蒙大拿大学校区唯一的集成生物分子扫描仪，几乎所有NIH支持的生物医学研究人员都使用它在广泛的常规分子生物学实验中进行定量成像：放射性标记生物分子的磷成像，凝胶中荧光染色的蛋白质和核酸的可视化，以及斑点和阵列中的化学荧光底物的检测。台风FLA9500包括与富士仪器类似的标准蓝、绿、红激光，并增加了多路复用两个荧光信号的新功能。替换仪器将继续由蒙大拿州生物技术中心使用既定的收费结构进行管理。购买台风FLA9500将使蒙大拿大学的生物医学研究人员继续获得高质量的磷光和荧光成像，以支持基础分子生物学在广泛的研究项目中的应用。

 DESCRIPTION (provided by applicant): This proposal seeks funding to purchase a Typhoon FLA9500 biomolecular imager (GE Healthcare Life Sciences) to replace a now-obsolete Fuji FLA3000G that has served our shared needs for 15 years. The Typhoon FLA9500 is a high-resolution high-sensitivity laser-scanning instrument with applications in phosphorimaging and fluorescence, chemifluorescence and colorimetric imaging. The current Fuji FLA3000G is the sole integrated biomolecular scanner on the University of Montana campus and is used by virtually all NIH-supported biomedical researchers for quantitative imaging in a broad range of routine molecular biology experiments: phosphorimaging of radiolabeled biomolecules, visualization of fluorescently stained proteins and nucleic acids in gels, and detection of chemifluorescent substrate in blots and arrays. The Typhoon FLA9500 includes standard blue, green and red lasers similar to those in the Fuji instrument and adds new capability for multiplexing two fluorescence signals. The replacement instrument will continue to be administered by the Montana Biotechnology Center using an established charge-back fee structure. Purchase of the Typhoon FLA9500 will provide biomedical researchers at the University of Montana continued access to high-quality phosphor and fluorescence imaging to support basic molecular biology applications in a wide range of research projects.