Multiplexed Pathogen Detection from Whole Blood for Rapid Detection of Sepsis

全血多重病原菌快速检测脓毒症 摘要 脓毒症，一种对感染的不良自身免疫反应，通常会导致生命危险 并发症是美国医院中死亡率和治疗费用最高的疾病。 在这项建议中，我们提出了一种新的微芯片来弥合病原体的现有缺口。 检测(灵敏度低，处理时间长)。 我们在拟议工作中的策略是对干的进行皮升体积的RT-LAMP反应 血液使用我们内部开发的双相反应来实现高度敏感的病原体 计数(1-3cfu/m L)。 该微孔阵列平台的实现潜在地克服了 目前血培养的敏感性和反应时间。我们预计这款设备将 大大有助于败血症的快速快速诊断。作为最终目标，我们预计这一点 平台将取代使用血液培养进行病原体检测。

Multiplexed Pathogen Detection from Whole Blood for Rapid Detection of Sepsis Abstract Sepsis, an adverse auto-immune response to an infection often causing life-threatening complications, results in the highest mortality and treatment cost of any illness in US hospitals. In this proposal we propose a novel microchip to bridge the existing gaps in the pathogen detection (poor sensitivity and long processing time). Our strategy in the proposed work is performing picolitre volume RT-LAMP reactions on dried blood using our in-house developed bi-phasic reaction to achieve a highly sensitive pathogen count (1-3 CFU/mL). The realization of this microwell array platform potentially overcomes the limitations in sensitivity and time of response of the current blood cultures. We expect that this device will greatly contribute to the faster rapid diagnosis of sepsis. As a final goal, we anticipate that this platform will replace the use of blood culture for pathogen detection.