Mechanisms and Models of Testis Specific Serine Kinase 6 (TSSK6)

摘要 睾丸特异性丝氨酸激酶家族(TSSK)的成员对精子成熟和 生育能力。然而，TSSK信号级联仍然定义不清。在这个家庭中，只有TSSK6是 被归类为癌症-睾丸基因(CT-基因)，因为其表达在广泛的范围内异常激活 癌症类型。我们发现TSSK6可以促进肿瘤前细胞的致癌行为。在……里面 在这个提议中，我们将描绘癌症中依赖TSSK6的信号通路，并确定如何 这一途径支持致瘤表型。此外，我们将开发细胞系、抗体和 为进一步研究这一特性不佳的激酶提供额外的试剂。这项工作将建立 TSSK6在肿瘤中异常表达和存在时发挥作用的分子机制 为未来的研究提供更多的实验工具。

Abstract Members of the Testis Specific Serine Kinases family (TSSK) are essential for sperm maturation and fertility. However, the TSSK signaling cascades remain poorly defined. Of this family, only TSSK6 is classified as a cancer-testis gene (CT-gene) as its expression is anomalously activated in a wide range of cancer types. We have found that TSSK6 can promote oncogenic behavior in pre-neoplastic cells. In this proposal, we will delineate the TSSK6-dependent signaling pathway in cancer and determine how this pathway supports tumorigenic phenotypes. In addition, we will develop cell lines, antibodies and additional reagents for further study of this poorly characterized kinase. This work will establish the molecular mechanism by which TSSK6 functions when anomalously expressed in tumors and present additional experimental tools for future investigations.