Novel Targets to Promote RGC Axon Regeneration: Insights from Unique RGC Cohorts

摘要 视网膜神经节细胞(RGC)轴突再生和再连接不良是 治疗眼外伤和青光眼等疾病。到目前为止，还没有需要修复的疗法 一旦造成损害，视神经就会受损。我们的新研究发现了一群RGC 有很高的再生能力。此外，我们现在发现了以前未被认识到的 不同的脂类促进轴突生长的能力。在目标1中，我们将使用高含量筛选 并从功能上测试各种候选基因促进轴突生长的能力。在AIM 2、我们将测定视网膜神经节细胞的脂类分布，并从功能上测试轴突生长促进作用。 精选的脂类。在目标3中，我们将使用在体视神经损伤模型来确定RGC轴突 接受各种处理的再生。找出可进一步增加研资局的新目标 轴突再生到大脑代表着一项重要的未来研究。从这些数据中获得的结果 研究将为开发未来修复退行性变的疗法提供宝贵的信息 视神经。

ABSTRACT Poor regeneration and reconnection of retinal ganglion cell (RGC) axons is a major obstacle for treating ocular trauma and diseases including glaucoma. There are as yet no therapies to repair optic nerve once the damage is done. Our new studies have discovered cohorts of RGCs that have a very high regenerative capacity. Furthermore, we now uncover previously unrecognized ability of distinct lipids to promote axon growth. In Aim 1, we will use High Content Screening and functionally test various candidate genes for their ability to promote neurite growth. In Aim 2, we will determine lipid profiles in RGCs, and functionally test neurite growth-promoting effects of select lipids. In Aim 3, we will use in vivo optic nerve injury model to determine RGC axon regeneration receiving various treatments. Identifying novel targets that further increase RGC axon regeneration to the brain represents a critical future study. Results obtained from these studies will provide invaluable information on developing future therapies to repair degenerated optic nerve.