UNMC Structural Biology Core

项目总结： 结构生物学(SB)核心的总体目标是将结构技术应用于分析 重要的生物大分子及其信号网络，提供疾病的基本知识 机制，推动研究和指导新疗法的合成。当衍射性晶体 可以生长然后原子图像的氨基酸侧链的排列可以在三维 获得。这些图像提供了可视化酶活性部位所需的原子细节，即DNA 转录因子的结合位置，并查看信号分子的蛋白质-蛋白质相互作用。功能 可以通过原子结构的测定来理解。在没有晶体的情况下，分子 大分子复合体、单个蛋白质的包膜及其总构象变化 配体结合可以用小角X射线散射(SAXS)来确定。翻译后修饰语 (PTM)改变蛋白质结构，对细胞信号转导很重要。可以用西方的分析方法来研究PTMS 等电聚焦(IEF)。Sb核心提供纳米毛细管电泳法(NanELP)免疫分析 用于PTMS的表征。将这些数据组合在一起，研究人员就可以学习如何修改 大分子的结构/功能关系；开发特定疗法的关键成分 副作用。

Project Summary: The overall goals of the Structural Biology (SB) core are to apply structural techniques to the analysis of important biological macromolecules and their signaling networks, to provide basic knowledge of disease mechanisms, drive research and direct the synthesis of novel therapeutics. When diffraction quality crystals can be grown then atomic images of the arrangement of amino acid side chains in three dimensions can be obtained. These images give the atomic detail needed to visualize the active sites of enzymes, see the DNA binding sites of transcription factors and view the protein-protein interactions of signaling molecules. Function can be understood through determination of atomic structures. In the absence of crystals, the molecular envelopes of macromolecular complexes, individual proteins and their gross conformational changes upon ligand binding can be determined using small angle X-ray scattering (SAXS). Posttranslational modifications (PTMs) alter protein structure and are important for cell signaling. PTMs can be studied using western analysis and isoelectric focusing (IEF). The SB core provides nanocapillary electrophoresis (nanoELP) immunoassays for the characterization of PTMs. When combined these data allow researchers to learn how to modify the structure/function relationships of macromolecules; a key component to developing specific therapies without side effects.