Integrated Genomics

整合基因组学共享资源 导演：克里斯蒂娜·哈林顿，博士 摘要/项目摘要 集成基因组学共享资源(IGSR)将先进的测序、微阵列和实时 时间聚合酶链式反应技术在已建立的核心环境中，具有交付高 优质的数据和全面的用户支持。作为基因组技术和专业知识的综合资源 在处理和处理广泛的RNA和DNA样本方面，IGSR能够为Knight提供建议 选择最符合他们的研究目标的基因组技术应用，然后有效地 支持这些选择。在目前的供资期间，IGSR大大扩大了高吞吐量 测序资源和服务，目前提供多种大规模并行测序(MPS) 选项，包括外显子组和面板测序、全基因组测序、RNA序列、芯片序列和甲基 SEQ，使用Illumina HiSeq 2500，升级的Illumina HiSeq 2000，用于2车道流动单元快速运行，以及 Illumina NextSeq 500。该设施为骑士成员提供：1)以一种 合理的成本；2)项目开发、实验设计和结果分析的内部咨询； 3)高度标准化和有质量控制的分析和数据准备，包括初始数据处理和 质量控制；4)基因组服务的快速周转时间；以及5)稳定的测序和 微阵列数据。最重要的是，IGSR拥有一支知识渊博、经验丰富的员工团队，以 支持和提供服务，并协助研究人员进行项目设计、赠款开发、分析 开发和研究出版。IGSR与骑士生物统计共享资源密切合作 以及在计算生物学和信息学方面的其他骑士和机构投资，以确保可访问和 确保数据存储安全，并制定和支持基因组数据处理、分析和分析的最佳做法 释义。IGSR将继续与骑士和其他OHSU利益攸关方合作，以确定和 实施新技术和分析以满足新的研究机会和不断发展的需求，包括 与骑士密切合作，提供新骑士早期检测所需的工具和支持 主动权。

INTEGRATED GENOMICS SHARED RESOURCE Director: Christina Harrington, Ph.D. ABSTRACT / PROJECT SUMMARY The Integrated Genomics Shared Resource (IGSR) brings together advanced sequencing, microarray, and real- time PCR technologies in an established core environment with demonstrated strengths in delivery of high- quality data and comprehensive user support. As an integrated resource for genomic technologies and expertise in the handling and processing of a wide range of RNA and DNA samples, the IGSR is able to advise Knight members on selection of genomic technology applications that best meet their research aims and then effectively support those choices. During the current funding period the IGSR has greatly expanded high-throughput sequencing resources and services, and currently provides a wide range of massively parallel sequencing (MPS) options, including exome and panel sequencing, whole genome sequencing, RNA Seq, ChIP Seq, and Methyl Seq, using an Illumina HiSeq 2500, an Illumina HiSeq 2000 upgraded for 2-lane flow cell rapid runs, and an Illumina NextSeq 500. The facility provides Knight members: 1) access to otherwise costly technology at a reasonable cost; 2) in-house consultation for project development, experimental design, and analysis of results; 3) highly standardized and quality-controlled assays and data preparation, including initial data processing and quality control; 4) rapid turnaround time for genomic services; and 5) a stable repository of sequencing and microarray data. Most importantly, the IGSR maintains a team of highly knowledgeable and experienced staff to support and deliver services and to assist researchers with project design, grant development, assay development, and research publication. The IGSR works closely with the Knight Biostatistics Shared Resource and other Knight and institutional investments in computational biology and informatics to insure accessible and secure data storage and to develop and support best practices for genomic data processing, analysis and interpretation. The IGSR will continue to work with the Knight and other OHSU stakeholders to identify and implement new technologies and assays that meet new research opportunities and evolving needs, including close collaboration with the Knight in providing tools and support needed by the new Knight early detection initiative.