NeuroImaging and Electrophysiological Facility

项目摘要/摘要：神经成像和电生理学设施(Nef) Cobre神经成像和电生理设备(Nef)已经发展成一种 神经可塑性研究中心和更大的波多黎各大学的ART资源 瑞科(UPR)生物医学研究社区。它还成功地继续加强了 所有用户的研究质量，以促进向有竞争力的联邦资金的过渡。我们 建议进一步扩大这些努力，使Nef成为一个自我可持续的形象和 功能强大的数据分析软件和全新的电生理仪器服务设施 设备和对现有Nef设备的升级，以满足我们不断增长的需求 以用户为基础。第一步(具体目标1)将是建立基于网络的云数据分析 目前在所有主要地点/校园为UPR科学家提供计算网络 进行神经科学研究。Nef将投资于新的计算机和软件工具 有必要向其所有用户提供现场和远程数据分析服务。第二步 (具体目标2)将继续升级和整合成像和电生理 在现有的Nef仪器中的应用。Nef将为现有用户和新用户提供 将电生理记录、动作电位的光学调制和 通过增加两个额外的电生理学装置来进行遗传方法 进行光遗传学研究。普遍定期审议将投资2000万美元加强这一目标。 在分子科学研究中心(MSRC)两层楼的翻新中 建立神经可塑性地板和最先进的动物设施。第三步 (具体目标3)在Nef的继续发展中，将进一步增加设备 通过购买超分辨率/TIRF实现灵活性和检测灵敏度(整体内部 荧光显微镜)显微镜系统和我们目前的一台共焦显微镜的升级 具有超分辨率/TIRF功能的系统。此外，增加了一个 脑电(EEG)套件将允许用户检测大脑活动和 量化这些变化与广泛的神经认知现象之间的关系。还有，尼夫 将继续并进一步加强对当代和下一代普遍定期审议科学家的培训 (具体目标4)通过我们非常成功的研讨会和研讨会系列。实现这些目标 四个目标将使Cobre Nef成为一个完全自我维持的神经成像研究 该中心是波多黎各和加勒比地区的设施和榜样。

Project Summary/Abstract: NeuroImaging and Electrophysiology Facility (NIEF) The COBRE Neuroimaging and Electrophysiology Facility (NIEF) has developed into a state-of- the-art resource for the Center for Neuroplasticity investigators and the larger University of Puerto Rico (UPR) biomedical research community. It has also successfully continued to strengthen the research quality of all its users to facilitate a transition toward competitive federal funding. We propose to further expand these efforts by making the NIEF a self-sustainable imaging and electrophysiology instrumentation service facility with powerful data analysis software and new equipment and upgrades to existing NIEF equipment in order to meet the needs of our growing user-base. The first step (Specific Aim 1) will be to establish a web-based cloud data analysis computing network available to UPR scientists located at all major sites/campuses currently conducting neuroscience research. The NIEF will invest in new computers and software tools necessary to provide onsite and remote data analysis services to all of its users. The second step (Specific Aim 2) will be to continue upgrading and integrating imaging and electrophysiological applications within existing NIEF instrumentation. The NIEF will provide existing and new users the ability to combine electrophysiological recordings, optical modulation of action potentials and genetic methods through the addition of two additional electrophysiology set-ups equipped for performing optogenetics. This aim will be strengthen by a 20 million dollar investment by the UPR in the renovation of 2 floors at the Molecular Sciences Research Center (MSRC) for the establishment of a Neuroplasticity floor and a state-of-the-art animal facility. The third step (Specific Aim 3) in the continued evolution of the NIEF will be to further increase the equipment flexibility and detection sensitivity by purchasing a Super-Resolution/TIRF (total internal fluorescent microscopy) microscopy system and the upgrading of one of our current confocal systems with Super-Resolution/TIRF capabilities. Moreover, the addition of an Electroencephalography (EEG) suite will allow users to detect changes in brain activity and quantify how these changes relate to a wide range of neurocognitive phenomena. Also, the NIEF will continue and further strengthen the training of current and next-generation UPR scientists (Specific Aim 4) through our highly successful seminar and workshop series. Fulfillment of these four aims will enable the COBRE NIEF to be a completely self-sustained neuroimaging research facility and a role model for Puerto Rico and the Caribbean.