Optical Imaging Tools for Elucidating the Roles of Anions and Anionic Modifications in Cellular Signaling

项目总结 阳离子通量已知可以驱动细胞信号事件，而阴离子则被认为具有如下功能 中和这些变化的反离子。但阴离子可能是动态的细胞信号吗？这种范式的转变 这个问题在很大程度上从化学生物学/生物无机的角度被忽视了，尽管事实是 这种阴离子调节失调与多种疾病有关，包括慢性疼痛、自闭症和癌症。这个 我们研究计划的长期目标是确定细胞来源、蛋白质靶标和信号转导作用 阴离子在健康和疾病中具有生物相关性，但相关技术仍不发达。对这件事 在这里，我们建议创建新的光学成像工具，它将允许将分子水平的图像构建到 负离子在生命系统中的实际作用方式、时间、地点和原因。我们的发现，不仅会 为阴离子在细胞信号转导中的作用，以及在表现中的作用提供了一个基本的理解， 相关疾病状态的诊断和治疗。

PROJECT SUMMARY Cation fluxes are known to drive cellular signaling events, whereas anions are considered to function as counterions to neutralize these changes. But could anions be dynamic cellular signals? This paradigm shifting question, in large part, has been overlooked from the chemical biology/bioinorganic perspective, despite the fact that anion dysregulation is implicated in a variety of diseases including chronic pain, autism, and cancer. The long-term goal of our research program is to identify the cellular sources, protein targets, and signaling roles of biologically relevant anions in health and disease, but the technologies to do so are still underdeveloped. To this end, here we propose to create new optical imaging tools that will allows to build a molecular level picture into the how, when, where, and why of what anions are actually doing in living systems. Our findings, will not only provide a fundamental understanding for the role of anions in cell signaling, but also in the manifestation, diagnosis, and treatment of associated diseased states.