Mechanisms of Contractile Network Assembly

项目摘要 细胞生物学中的一个基本问题是如何产生适当水平的细胞收缩性 来驱动细胞和组织的形态发生。我们知道细胞必须协调收缩活动， 实现动态移动。我们不知道它们是如何产生和调节右心室的收缩力的 在正确的时间地点。肌球蛋白2丝是所有肌球蛋白中产生力的主要马达蛋白。 细胞和组织。光学显微镜的最新进展使我们能够观察到肌球蛋白2 以前所未有的空间和时间分辨率研究活细胞的动态。我们观察到 亚细胞水平的复杂性，这是我们在几十年的体外实验中从未考虑或预料到的。 问题研究这项建议使用高分辨率成像沿着细胞和分子的工具箱， 分析细胞构建肌动球蛋白2收缩结构的机制， 让它们适应细胞过程，并解构它们。最近的发现清楚地表明， 经过几十年的研究，肌球蛋白2仍然隐藏着我们必须揭开的秘密， 了解细胞收缩性。我们预计这项工作的完成将回答目前的问题。 问题，并产生重要的新问题。

Project Summary A fundamental question in cell biology is how the proper level of cell contractility is generated to drive cell and tissue morphogenesis. We know that cells must coordinate contractile events to enable dynamic movements. We do not know how they generate and tune contractility in the right place at the right time. Myosin 2 filaments are the dominant force-generating motor proteins in all cells and tissues. Recent advances in light microscopy have enabled us to observe myosin 2 dynamics in living cells at unprecedented spatial and temporal resolution. We have observed complexity at the subcellular level that we never considered or anticipated from decades of in vitro studies. This proposal uses high-resolution imaging along with a toolbox of cellular and molecular assays to dissect the mechanisms by which cells build actomyosin 2 contractile structures, tune them to fit the cellular process, and deconstruct them. Recent discoveries have clearly shown that after decades of investigation, myosin 2 is still holding secrets that we must uncover to fully understand cellular contractility. We anticipate the completion of this work will answer current questions and yield important new ones.