Methods for Fast and Efficient Oxygen Imaging

摘要 肿瘤的间歇性或急性/周期性缺氧，频率在每分钟几个周期到几个小时之间，是 受到越来越多的关注，因为据报道，这种类型的缺氧对肿瘤有影响 通过增加促生存通路的表达来增加恶性程度和治疗耐药性。快氧 急性肿瘤缺氧的测量需要影像方法。脉冲电子顺磁 磁共振成像(PEPRI)是一种很有前途的提供三维偏氧分压(PO2)图的工具 在活体组织和肿瘤中协助肿瘤生物学、血流灌注、药物开发和 放射治疗。单点成像(SPI)，一种由美国国家癌症研究所开发的采集技术 (NCI)是可用于氧气图像采集的pEPRI方法的子集。它提供高分辨率、 高保真图像，但由于需要单独获取每个k空间点，因此速度较慢。在当前 项目，我们的目标是通过利用先进的组合来提高SPI的图像采集速度 硬件和深度学习。这将使成像速度提高许多倍，而不会影响 图像质量。这些进展将在纤维肉瘤肿瘤的小鼠模型中进行测试。这个项目将带来一个 NIH-开发的技术达到了商业水平。我们的长期目标是暗示先进的硬件和 将氧气成像软件技术应用于临床，辅助氧气引导的肿瘤治疗。

Abstract Intermittent or acute/cyclic hypoxia in tumors, with frequencies between a few cycles per minute to hours, is receiving increased attention because this type of hypoxia has been reported to have an influence on tumor malignancy as well as treatment resistance via increased expression of pro-survival pathways. Fast oxygen imaging methods are needed for the measurement of acute tumor hypoxia. Pulse electron paramagnetic resonance imaging (pEPRI) is a promising tool to provide three-dimensional partial oxygen pressure (pO2) maps in live tissues and tumors to assist with advanced studies of tumor biology, perfusion, drug development, and radiation treatment. Single point imaging (SPI), an acquisition technique developed at National Cancer Institute (NCI), is a subset of pEPRI methods that can be used for oxygen image acquisition. It provides high-resolution, high-fidelity images but is slow due to the need for acquiring each k-space point individually. In the current project, our goal is to improve the image acquisition speed of SPI by utilizing a combination of advanced hardware and deep learning. This will improve the imaging speed by many folds without compromising the image quality. These advances will be tested in a mouse model of fibrosarcoma tumor. This project will bring an NIH-developed technology to the commercial level. Our long-term goal is to imply the advanced hardware and software technologies of oxygen imaging to clinics to assist with oxygen-guided tumor treatments.