Core C TEST IT

皮肤形成与环境的主要接口，角质形成细胞是天生的关键成分 免疫系统。健康皮肤的紊乱会通过皮肤免疫反应导致炎症。 因此，皮肤的免疫学是SBDRC的一个重要焦点。 以“微环境”为主题。皮肤免疫系统需要一套互动的先天免疫和获得性免疫 可以进行原位研究、拆分进行单细胞水平研究或重建以重建的细胞 皮肤的免疫状态。转化性和实验性皮肤测试和免疫的目标 溯源(TEST IT)的核心是支持和推动SBDRC科学家对人类和 健康和疾病中的小鼠皮肤免疫反应。核心将分离各种免疫细胞从 健康和患病的皮肤，包括在单细胞水平上，并为表征提供准备服务 直接或在功能分析中检测这些细胞。使用Vectra多光谱成像设备，Core将提供帮助 在设计允许对人和小鼠皮肤样本、外植体和工程化皮肤样本进行现场监测的实验中 皮肤。核心将为有效地从皮肤和血液中扩增免疫细胞提供指导 皮肤组织工程与形态学与3D重组人皮肤或植入物的重组 (杆芯)。在重建之前，细胞可以用基因的产物进行基因操作 工程、换能器和纳米技术(进入)核心。这些经过改造的角质形成细胞免疫细胞 然后，模型将可用于进一步的免疫评估。测试IT的一个独特且非常新颖的方面 核心将是为活体监测提供皮肤挑战设施。这将通过一个 专门的临床单位和工作人员测试暴露在影响皮肤免疫的环境因素中， 无论是以潜在有害的方式，如紫外线/紫外线，还是以有益的方式，如局部用药 抑制免疫反应的药物。核心将监督组织获取计划(TAP)， 获取档案样本，以及新鲜皮肤、血液和其他样本(包括通过体内 皮肤挑战设施)用于具有安全记录的中央生物储存库；编码的、已识别的组织被分发 提供给科学家，供个别实验室或服务核心使用。核心还将保持一份健康的 愿意参加IRB批准的皮肤挑战的志愿者和皮肤病患者 实验。这些翻译研究支持机械性调查，并提供概念验证数据。 核心还侧重于培训免疫皮肤病的研究社区，提供专业知识 设计涉及皮肤免疫系统的小鼠和人类实验，并在 免疫皮肤学、免疫治疗学和免疫监测。这是一个新的核心，专注于支持 研究人员进行高级和高度翻译的免疫皮肤研究。学习能力受挫 受控的人类皮肤将极大地促进翻译研究。

The skin forms a primary interface with the environment, with the keratinocyte a critical component of the innate immune system. Perturbation of healthy skin can lead to inflammation through cutaneous immune responses. As such, the immunology of skin is an important focus of a SBDRC with “The Keratinocyte and its Microenvironment” as its theme. he skin immune system entails an interactive set of innate and adaptive immune cells that can be studied in situ, taken apart for studies at the single cell level, or reconstructed to recreate immune conditions of the skin. The objective of the Translational and Experimental Skin Testing and Immune Tracing (TEST IT) Core is to support and promote the research of SBDRC scientists interested in human and murine cutaneous immune responses in health and disease. The Core will isolate various immune cells from healthy and diseased skin, including at the single cell level, and offer preparative services for the characterization of these cells directly or in functional assays. Using Vectra multispectral imaging equipment, the Core will assist in designing experiments allowing in situ monitoring of human and mouse skin samples, explants and engineered skin. The Core will provide guidance for the efficient expansion of immune cells from skin and blood to be recombined with 3D reconstituted human skin or explants by the Skin Tissue Engineering and Morphology (STEM Core). Prior to reconstruction, cells can be genetically manipulated with products from the Gene Engineering, Transduction and Nanotechnology (GET iN) Core. These engineered keratinocyte-immune cell models will then be available for further immune assessment. A unique and highly novel aspect of the TEST IT core will be to provide a skin challenge facility for in vivo monitoring. This will be accomplished through a dedicated clinical unit and staff to test exposure to environmental agents that affect skin immunologically, whether in a potentially deleterious way, such as ultraviolet/UV light, or in a beneficial way, such as topical medications that suppress immune responses. The Core will oversee the Tissue Acquisition Program (TAP), which obtains archival samples, as well as fresh skin, blood, and other samples (including through the in vivo skin challenge facility) for a central biorepository with secure records; coded, deidentified tissues are distributed to scientists for use in individual laboratories or the service Cores. The Core will also maintain a roster of healthy volunteers and individuals with skin disease who are willing to participate in IRB-approved, skin challenge experiments. These translational studies support mechanistic investigations and provide proof-of-concept data. The Core also focuses on training the research community about immunodermatology, offering expertise in designing mouse and human experiments involving the skin immune system and generating course material in immunodermatology, immunotherapeutics, and immune monitoring. This is a novel core that focuses on enabling investigators to perform advanced and highly translational immune skin research. The ability to study perturbed human skin in a controlled manner will greatly facilitate translational research.