SBIR TOPIC 107 REAGENTS FOR IMMUNOLOGIC ANALYSIS OF NON- MAMMALIAN AND UNDERREPRESENTED MAMMALIAN MODELS

仓鼠是包括流感和SARS-CoV-2在内的几种传染病的成熟模型。仓鼠试剂的缺乏阻碍了病毒发病机制、宿主免疫和疫苗开发的临床前研究。本提案的目的是生产和测试针对10种细胞因子和趋化因子以及4种T细胞表面标志物的单克隆抗体，用于表征呼吸道感染仓鼠模型中的细胞因子应答并检查T细胞活化。该项目的完成将提供高亲和力的单克隆抗体，使研究人员能够区分先天性和适应性炎症细胞因子和趋化因子反应以及感染性疾病仓鼠模型中的活化T细胞，记忆T细胞，组织驻留记忆T细胞和调节性T细胞。

The hamster is a well-established model for several infectious diseases including influenza and SARS-CoV-2. Lack of hamster reagents has hampered pre-clinical studies of viral pathogenesis, host immunity, and vaccine development. The objectives of this proposal are to produce and test monoclonal antibodies against 10 cytokines and chemokines and 4 T cell surface markers for characterizing cytokine responses and examining T cells activation in the hamster models of respiratory infections. Completion of this project will provide high-affinity monoclonal antibodies that allow investigators to distinguish innate and adaptive inflammatory cytokine and chemokine responses as well as activated T cells, memory T cells, tissue resident memory T cells, and regulatory T cells in the hamster models of infectious diseases.