DEVELOPMENT OF FERRET REAGENTS FOR USE IN THE CHARACTERIZATION OF IMMUNE RESPONSES TO RESPIRATORY INFECTIONS IN THE FERRET MODEL.

开发雪貂试剂，用于表征雪貂模型中呼吸道感染的免疫反应。

• 批准号: 10244744
• 负责人: TORI RACE
• 金额: $ 57.28万
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-07-01 至 2022-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10244744
• 关键词: Antibodies; Antigens; Cells; Contractor; Ferrets; Generations; Goals; Hybridomas; Immune; Immune response; Immunization; Immunologics; Influenza A virus; Methods; Middle East Respiratory Syndrome Coronavirus; Modeling; Monoclonal Antibodies; Mus; Proteins; Reagent; Recombinants; Respiratory Tract Infections; SARS coronavirus; Sampling; Specificity; Techniques; Validation; cytokine; human model; respiratory virus; response

Ferrets represent excellent models of human respiratory viruses such as influenza A virus, SARS-CoV and MERS-CoV. The goal of this proposal is to generate antibodies for analyzing immune cells and cytokine responses during respiratory infections in ferrets. The main focuses are immune cell markers and cytokines produced by these cells during immune responses. The contractor plans to use standard hybridoma techniques to generated monoclonal antibodies will then undergo extensive immunological characterization using various methods to show the specificity to recombinant immunogens as well as selected ferret samples.

雪貂是甲型流感病毒、SARS-CoV和MERS-CoV等人类呼吸道病毒的优秀模型。本提案的目的是产生抗体分析免疫细胞和细胞因子反应在呼吸道感染雪貂。主要的焦点是免疫细胞标记和细胞因子产生的这些细胞在免疫反应。承包商计划使用标准杂杂瘤技术生成单克隆抗体，然后使用各种方法进行广泛的免疫学表征，以显示重组免疫原和选定的雪貂样本的特异性。