Analysis of the Yeast Cell Cycle Transcriptional Program

描述（由申请人提供）：细胞周期对照品被组织成一个循环，因此周期可能会反复发生。这些控制有几种：转录控制，蛋白水解控制，蛋白激酶控制，等微阵列技术的发展，现在已经使得它更容易和更有效地调查各种转录控制。事实上，关于细胞周期转录调控的微阵列研究已经提供了丰富的数据。在这个提议中，微阵列技术将被用来继续研究酵母细胞周期的转录调控。特别是，将寻求细胞周期控制的循环性质的概述。在目标1中，我们将完成将细胞周期调控基因分配到其调控转录因子的任务。在目标2中，我们将发现细胞周期基因表达的时间是如何确定的，我们将定义周期中组合控制的程度和机制。在目标3中，我们将测试组蛋白乙酰化状态的振荡是细胞周期转录控制的主要机制这一假设。

DESCRIPTION (provided by applicant): Cell cycle controls are organized into a loop, so that cycles may occur over and over again. These controls are of several kinds: transcriptional controls, proteolytic controls, protein kinase controls, etc. The development of microarray technology has now made it far easier and more efficient to investigate all kinds of transcriptional controls. Indeed, microarray studies on the transcriptional regulation of the cell cycle have provided a wealth of data. In this proposal, microarray technology will be used to continue the study of the transcriptional control of the yeast cell cycle. In particular, an overview of the circular nature of cell cycle controls will be sought. In Aim 1, we will complete the task of assigning cell cycle regulated genes to their regulating transcription factors. In Aim 2, we will find out how the time at which cell cycle genes are expressed is determined, and we will define the extent and mechanisms of combinatorial control in the cycle. In Aim 3, we will test the hypothesis that an oscillation in the state of histone acetylation is a major mechanism of cell cycle transcriptional control.