Colorado Gene Array Core

科罗拉多州基因阵列核心将专注于创建可用于开发的数据库 小鼠和大鼠脑的表达QTL(EQTL)图谱。我们目前正在扩充大脑的数据库 BxD RL小鼠品系雄性和雌性的基因表达谱。拟议的研究将 从31个BxH/HxB大鼠RL品系的大脑中生成一个类似的基因表达谱数据库，并 祖代品系(雄株和雌株)，以及大鼠品系的微卫星和SNP基因分型 将与田纳西大学的罗伯威廉姆斯博士合作表演。此信息将允许 大鼠脑基因表达的eQTL定位及饮酒相关QTL的定位 菌株。当与复杂性状的QTL分析相结合时，eQTL的产生有助于 识别这些性状的候选基因，核心将使用此程序和生成的数据 确定导致大鼠焦虑和其他酒精相关行为的候选基因。这个 脑基因表达数据的可用性和小鼠和大鼠脑转录组的eQTL定位将 制作一种新颖而独特的资源，供酒精中毒调查人员使用。核心也将执行基因 为合作的INIA调查人员提供表达分析和定量RT-PCR，并提供访问 对生成的数据进行标准化、统计分析和eQTL搜索的方法。

The Colorado Gene Array Core will focus on the creation of databases that can be used for developing expression QTL (eQTL) maps for mouse and rat brain. We are currently expanding the database of brain gene expression profiles for males and females of the BxD Rl mouse strains. The proposed studies will generate a similar database of gene expression profiles from brains of 31 Rl strains of BxH/HxB rats and the progenitor strains (males and females separately), and microsatellite and SNP genotyping of the rat strains will be performed in collaboration with Dr. RobWilliams, University of Tennessee. This information will allow for eQTL mapping for rat brain gene expression as well as mapping of QTLs for alcohol drinking in the rat strains. When combined with QTL analysis of complex traits, the generation of eQTLs facilitates the identification of candidate genes for these traits, and the Core will use this procedure and the generated data to identify candidate genes that contribute to anxiety and other alcohol related behaviors in the rats. The availability of brain gene expression data and eQTL mapping of the mouse and rat brain transcriptome will produce a novel and unique resource for use by alcoholism investigators. The Core will also perform gene expression analysis and quantitative RT-PCR for collaborating INIA investigators, and provide access to methodology for normalization, statistical analysis and eQTL searches of the generated data.