Struture snd Dynamic Determinants of Ion Channel Assembly by Adapter Proteins

描述（由申请人提供）：囊性纤维化是一种毁灭性的、慢性的、进行性的、经常致命的遗传性疾病，尤其在肺部表现出来。主要原因是囊性纤维化跨膜传导调节因子（CFTR）在跨细胞膜离子运输中的异常。CFTR是几种上皮组织中主要的氯离子转运体。na +/H+交换调节因子（NHERF）和ezrin两种适配蛋白调节CFTR的细胞表面浓度，组织CFTR与信号蛋白网络的大分子相互作用，实现高效转导，最终控制氯离子转运的强度。本研究的目的是确定适配蛋白协同调节CFTR大分子组装的分子机制。要验证的中心假设是NHERF是一种信号传感器，其特定的分子内相互作用由ezrin调节，控制NHERF组装CFTR的能力。通过研究多价转接头蛋白组装CFTR大分子复合物的结构、能量学和形成动力学，本研究将定量分析转接头蛋白相互作用组装CFTR通道的分子机制。对CFTR与大分子相互作用的分子理解是开发治疗囊性纤维化的治疗策略的基本要素。

DESCRIPTION (provided by applicant): Cystic fibrosis is a devastating, chronic, progressive, and frequently fatal genetic disease that is particularly manifest in the lungs. The major cause is an abnormality in ion transport across cell membranes by the cystic fibrosis transmembrane conductance regulator (CFTR). CFTR is the dominant chloride ion transporter in several epithelial tissues. Two adapter proteins-Na+/H+ exchanger regulator factor (NHERF) and ezrin-regulate the cell surface concentrations of CFTR, organize the macromolecular interactions of CFTR with a network of signaling proteins for efficient transduction, and ultimately control the strength of chloride ion transport. The goal of this research is to determine the molecular mechanisms by which adapter proteins work in coordination to regulate the macromolecular assembly of CFTR. The central hypothesis to be tested is that NHERF is a signal transducer whose specific intra-molecular interactions, which are modulated by ezrin, control the ability of NHERF to assemble CFTR. By studying the architecture, energetics, and dynamics of the formation of CFTR macromolecular complexes assembled by multivalent adapter proteins, this research will provide a quantitative analysis of the molecular mechanisms by which adapter proteins interact to assembly CFTR channels. A molecular understanding of the macromolecular interactions with CFTR is an essential element for developing a therapeutic strategy for the cure of cystic fibrosis.