Shared Resource in Cellular Imaging

摘要 亨廷顿医学研究院(HMRI)细胞成像核心的愿景是 为调查人员提供获取尖端技术的途径和专家技术支持 细胞解剖学和生理学的显微观察和分析 我们多学科生物医学研究的方方面面。我们当前成像中的一个主要差距 系统在图像质量、吞吐量和可访问性之间取得了平衡。由于 这些限制，HMRI的研究人员需要将他们的Brightfield和 免疫荧光需要昂贵而繁忙的核心设施。在这里，我们建议克服 这些工作流程和高成本限制通过提出一体式荧光显微镜来实现， BZ-X800，具有多种功能，将加强各个实验室的细胞生物学项目 核磁共振成像。该仪器将被安置在细胞成像核心设施中， 新建的研究大楼的二楼，并将加快研究，由5 NIH R01授予稳健和定量的荧光和明场成像。核心意志 将由多名调查人员共享，并将加强本科生、STEM和 HMRI的博士后教育项目。这将是实现持续发展目标的关键 NIH资助的项目，以及计划中的未来项目。这款一体式显微镜将使 我们的研究以前所未有的速度，并为理解和 精神病、神经病和心脏病的治疗。

Abstract The vision of the Cellular Imaging Core of the Huntington Medical Research Institutes (HMRI) is to provide investigators access to cutting-edge technology, and expert technical support for microscopic observation and analysis of cellular anatomy and physiology that is essential for diverse aspects of our multidisciplinary biomedical research. A major gap in our current imaging systems is acquiring a balance between image quality, throughput, and accessibility. Due to these limitations, researchers at HMRI need to outsource their brightfield and immunofluorescence needs to costly and busy core facilities. Here we propose to overcome these workflow and high-cost limitations by proposing an all-in-one fluorescence microscope, BZ-X800 that has multiple features and will strengthen the cell biology projects in various labs at HMRI. The instrument will be housed in the Cellular Imaging Core facility designated to the second floor of the newly built research building and will accelerate research supported by 5 NIH R01 grants, on robust and quantitative fluorescence and brightfield imaging. The Core will be shared by multiple investigators and will strengthen the undergraduate, STEM and postdoctoral educational programs at HMRI. It will be critical for meeting the goals of ongoing NIH-funded projects, as well as planned future projects. This all-in-one microscope will facilitate our research at unprecedented speeds, and forge new avenues for understanding and treatment of psychiatric, neurological, and heart disease.