Neuropeptidergic Inhibition of Spinal Pain Transmission

描述（由申请人提供）：独立科学家奖（K02）候选人寻求5年的薪水支持，以提供释放时间来研究神经肽在介导炎症和神经性疼痛中的作用。他的综合方法包括药理学、行为学和分子研究。该候选人在加州大学圣地亚哥分校获得博士学位，曾与加州大学旧金山分校的Allan Basbaum合作，现任杜兰大学药理学副教授。他发表了28篇原创文章（25篇为第一/最后作者），7篇特邀评论，并获得NRSA， first (R29)， R21和R01资助，研究疼痛和镇痛。他广泛的职业目标包括：1；将他目前的R01扩展到一个精心设计的长期研究项目，以产生一种新的慢性疼痛药物疗法（例如NPY受体激动剂）；2. 与导师的持续互动将加深他对慢性疼痛的生理、细胞和生化机制以及神经肽的抑制作用的理解；和3。在分子生物学、病毒转染和条件转基因小鼠技术的应用方面继续开展合作和培训，以便为慢性疼痛研究中的重要问题开发精确和有针对性的方法。杜兰大学仍然拥有丰富的智力成长和独立的教育机会，并为泰勒博士提供了出色的资源，包括实验室空间、设备、动物园内的行为套房和内部研究资金。杜兰大学认为泰勒博士是其对研究的坚定承诺的组成部分，并于2004年授予他早期终身教职。根据K02基金，该机构保证泰勒博士85%的时间将用于研究和职业发展活动。研究计划的重点是总体假设炎症或感觉神经损伤减少背角NPY信号的突触前和突触后成分，从而促进前感觉神经传递，最终增加异位性疼痛和痛觉过敏。目的1将使用新的受体亚型选择性拮抗剂和缺失突变小鼠来确定Y1或Y2受体对NPY作用的贡献。目的2将把损伤引起的行为变化与NPY释放和Y1受体表达联系起来。Aim #3将使用微透析和免疫组织化学来确定NPY是否抑制P物质释放和背角伤害反应神经元。

DESCRIPTION (provided by applicant): The candidate for an Independent Scientist Award (K02) seeks five years of salary support to provide release time to study the role of neuropeptides in mediating inflammatory and neuropathic pain. His integrative approach includes pharmacologic, behavioral and molecular studies. The candidate obtained a Ph.D. at UCSD, worked with Allan Basbaum at UCSF, and is now an Associate Professor of Pharmacology at Tulane University. He has published 28 original articles (25 as first/last author) plus 7 invited reviews and received NRSA, FIRST (R29), R21 and R01 grants to study pain and analgesia. His broad career goals include: 1. Extension of his current R01 into a well-conceived, long-term research program that yields a new pharmacotherapy for chronic pain (e.g. an NPY receptor agonist); 2. Continued interactions with mentors that will foster his deeper understanding of the physiological, cellular and biochemical mechanisms of chronic pain and its inhibition by neuropeptides; and 3. Continued collaborations and training in the utilization of molecular biological, viral transfection, and conditional transgenic mouse technologies so as to develop precise and targeted approaches to important questions in chronic pain research. Tulane remains rich in educational opportunities for intellectual growth and independence, and provides outstanding resources to Dr. Taylor including laboratory space, equipment, behavioral suites in the vivarium, and internal research funding. Tulane considers Dr. Taylor to be an integral part of its strong commitment to research, and granted him early tenure in 2004. Contingent upon K02 funding, the institution provides assurances that 85% of Dr.Taylor's time will be set aside for research and career development activities. The Research Plan focuses on the overall hypothesis that inflammatory or sensory nerve injury decreases presynaptic and postsynaptic elements of NPY signaling at the dorsal horn, thereby facilitating pronociceptive neurotransmission, and ultimately increasing allodynia and hyperalgesia. Aim #1 will determine the contribution of Y1 or Y2 receptors to the actions of NPY using new receptor subtype- selective antagonists, and deletion mutant mice. Aim #2 will correlate injury-induced changes in behavior with NPY release and Y1 receptor expression. Aim #3 will use microdialysis and immunohistochemistry to determine whether NPY inhibits substance P release and dorsal horn nociresponsive neurons.