SIMULTANEOUS ELECTRICAL AND OPTICAL RECORDINGS IN HEART

描述(改编自申请者的摘要)：这个项目将发展 一种可用于测量细胞外的透明电极阵列 电压和跨膜电压敏感染料的同时荧光 来自心脏的相同位置。电极将使用以下材料制造 显微光刻技术与氧化锡薄膜沉积。 第一个电极阵列将被制造并用于量化 氧化锡薄膜的电学和光学性质是重要的 用于电学和光学测量。那么电极阵列将会是 作为同时电学和光学的原型制造 测量。这些阵列将被用来证明电极 可同时测量心脏电信号和光信号 来自动脉灌注的兔心中相同的位置。这些阵列将 用于获得初步数据，使S.Knisley博士能够提交 新的R01拨款申请，其目标是验证 心律失常部位常用的细胞外记录方法 情况包括阻塞、波前碰撞、再入和多次 纤颤的小波。这将改善对 细胞外信号，这反过来将允许更好地确定 患者消融手术中心律失常的起源部位和 允许更好地从细胞外确定心律失常的机制 录音。但首先有必要确定是否有可能 为了制造电极阵列来测量细胞外电压和 跨膜电压敏感染料荧光同时从 心脏中的相同位置。这个项目有很大的风险，因为他们 不知道氧化锡之间界面的电化学性质 心脏组织，他们不知道锡的光学性质 氧化物，他们不知道人们是否能把一个阵列结构组合在一起 包含所有必要的电学和光学属性。

DESCRIPTION (Adapted from applicant's abstract): This project will develop a transparent electrode array that can be used to measure extracellular voltages and transmembrane voltage-sensitive dye fluorescence simultaneously from the same locations in the heart. Electrodes will be fabricated using microphotolithographic techniques and thin film deposition of tin oxide. The first electrode arrays will be fabricated and used to quantify the electrical and optical properties of the tin oxide film that are important for the electrical and optical measurements. Then electrode arrays will be fabricated as prototypes for the simultaneous electrical and optical measurements. These arrays will be used to demonstrate that the electrodes can simultaneously measure cardiac electrical signals and optical signals from the same locations in arterial-perfused rabbit hearts. The arrays will be used to obtain preliminary data that will allow Dr. S. Knisley to submit a new R01 grant application, which will have the objective of validating the commonly-used extracellular recording method at the sites of arrhythmic conditions including block, wavefront collision, reentry and multiple wavelets of fibrillation. This will improve the interpretation of extracellular signals, which in turn will allow better determination of sites of origins of arrhythmias for ablation procedures in patients and allow better determination of mechanisms of arrhythmias from extracellular recordings. But first it is necessary to determine whether it is possible to fabricate an electrode array to measure extracellular voltages and transmembrane voltage-sensitive dye fluorescence simultaneously from the same locations in the heart. This project has significant risk since they don't know the electrochemical properties of the interface between tin oxide and the heart tissue, they don't know the optical properties of the tin oxide and they don't know if one can put together an array structure that contains all of the necessary electrical and optical properties.