Hypoxia: an activator of neuroimmunity

描述(由申请人提供)：疾病行为被经典地定义为一组协调的非特异性行为改变，包括食欲丧失、性欲下降、运动活动以及对物理和社会环境的兴趣，这些都是对感染的反应。我们实验室的最新研究表明，急性缺氧会导致小鼠丧失社会探索能力，这表明非传染性刺激可以激活神经免疫系统，刺激疾病行为。急性缺氧发生在许多类型的事故和多种疾病状态中，但对急性缺氧的免疫行为激活和恢复知之甚少。我们令人兴奋的新数据表明，急性缺氧会导致社会探索能力的丧失和大脑IL-12的快速上调。重要的是，在缺乏IL-1受体/Toll样受体超家族信号的小鼠中，这种急性缺氧诱导的疾病行为显著缩短。在瘦素受体缺陷小鼠中，我们已经证明急性缺氧性疾病持续时间更长，并通过皮下注射IL-1受体拮抗剂(IL-1RA)来改善。总而言之，这些数据表明，急性缺氧触发了基于大脑的先天免疫系统的激活。这项应用的目的是检验一种假说，即急性缺氧激活神经免疫系统的IL-1臂，在缺氧恢复期间导致疾病行为。该项目的长期目标是了解急性缺氧后免疫行为恢复的机制，并开发预防和/或加速从急性缺氧的免疫行为后果中恢复的策略。在目标1中，我们将定义在缺氧恢复过程中经历的急性缺氧引起的疾病行为，并确定它们是否由促炎细胞因子IL-12介导。在目标2中，我们将确定瘦素是否是快速解决急性缺氧引起的社会探索丧失的关键，并发现肥胖对急性缺氧诱导的疾病行为恢复的影响。在目标3中，我们将使用一种以IL-1RA为特征的药理学方法来加速急性缺氧诱导的疾病行为的恢复，确定IL-1诱骗受体(IL-1R2)在急性缺氧恢复中的重要性，并确定依赖钒的脑组织IL-1RA和/或IL-1R2的上调是否可以提供急性缺氧的保护。需要这些研究来确定新的目标，以减轻急性缺氧症患者的痛苦。

DESCRIPTION (provided by applicant): Sickness behavior has been defined classically as a coordinated set of nonspecific behavioral modifications including loss of appetite, libido, motor activity and interest in the physical and social environment that occur in response to infection. New research from our laboratory shows that acute hypoxia induces loss of social exploration in mice, demonstrating that non-infectious stimuli can activate the neuroimmune system and stimulate sickness behavior. Acute hypoxia occurs in many types of accidents and in a host of disease states, yet little is known about immunobehavioral activation and recovery from acute hypoxia. Our exciting new data demonstrate that acute hypoxia elicits loss of social exploration and rapid up-regulation of brain IL-12. Importantly, this acute hypoxia-induced sickness behavior is dramatically shortened in mice deficient in IL-1 receptor/Toll-like receptor superfamily signaling. In leptin receptor defective mice, we have shown that acute hypoxia-induced sickness is longer lasting and ameliorated by subcutaneous administration of IL-1 receptor antagonist (IL-1RA). Together, these data indicate that acute hypoxia triggers activation of the brain-based innate immune system. The objective of this application is to examine the hypothesis that acute hypoxia activates the IL-1 arm of the neuroimmune system causing sickness behavior during hypoxia recovery. The long-term goal of this project is to understand the mechanisms of immunobehavioral recovery from acute hypoxia and to develop strategies that prevent and/or speed recovery from the immunobehavioral consequences of acute hypoxia. In Objective #1, we will define the acute hypoxia-induced sickness behaviors experienced during hypoxia recovery and determine if they are mediated by the pro-inflammatory cytokine IL-12. In Objective #2, we will ascertain if leptin is essential to rapidly resolving loss of social exploration due to acute hypoxia and discover the impact of obesity on recovery from acute hypoxia-induced sickness behaviors. In Objective #3, we will use a pharmacologic approach featuring IL-1RA to speed recovery from acute hypoxia-induced sickness behaviors, determine the importance of the IL-1 decoy receptor (IL-1R2) to acute hypoxia recovery and ascertain if vanadium-dependent up-regulation of brain IL-1RA and/or IL- 1R2 can provide protection from acute hypoxia. These studies are needed to define new targets in order to alleviate suffering in those afflicted by acute hypoxia.