Protein Expression Core

东北生物防御中心应用范围内的许多工程都需要高质量的纯化重组 蛋白质和伪型病毒用于他们的研究。蛋白质表达核心的目标是促进 在本申请的不同主题中提出的调查性、发展性和先导性研究。的工作人员 核心将协助研究人员将他们的研究转化为质量控制的标准分析， 疫苗或疗法。工作人员将与调查人员合作开发蛋白质的表达系统 他们的研究需要使用商业载体或定制的表达载体。该设施将 提供完整的蛋白质表达和纯化服务。这将包括进入成熟的 沃兹沃斯中心的细菌表达能力；利用哺乳动物的专门能力， 阿尔伯特·爱因斯坦医学院的昆虫表达和体外翻译；以及制作 耶鲁大学专门设计的假型VSV病毒，极大地促进了中和抗体的分析 大学。这三个服务组件由各自领域的专家领导，将允许快速 为研究项目提供试剂，并为NBC提供灵活性，以支持广泛的 满足现有需求，并迅速对首席调查员不断变化的要求作出反应 改变NIAID的命令。此外，核心将保持稳定的蛋白质和表达库存 菌株，以便在紧急情况下，这些材料的生产将迅速。

Many projects within the Northeast Biodefense Center application require high-quality purified recombinant proteins and pseudotyped virus for their research. The goal of the Protein Expression Core is to facilitate the investigative, developmental, and pilot studies proposed in the various themes of this application. The staff of the Core will assist investigators in the translation of their studies to quality controlled standard assays, vaccines or therapeutics. Staff will work with investigators to develop expression systems for the proteins required for their studies either using commercial vectors or customized expression vectors. The facility will provide a complete protein expression and purification service. This will include access to well established bacterial expression capabilities at the Wadsworth Center; specialized capabilities exploiting mammalian, insect expression, and in vitro translation at the Albert Einstein College of Medicine; and the production of specially designed pseudotyped VSV viruses that greatly facilitate analysis of neutralizing antibodies at Yale University. These three service components, headed by experts within their field, will allow the rapid provision of reagents for research projects and provide the NBC with the flexibility to support the wide range of existing needs and to respond rapidly to the changing requirements of principal investigators and changing NIAID mandates. In addition, the Core will maintain stable stocks of proteins and expression strains so that in a time of emergency, production of these materials will be rapid.