Protein Microarray

蛋白质微阵列核心的目标是为研究人员提供高通量的基因组克隆 蛋白质微阵列可用于发现疫苗和血清诊断抗原的平台。 制造整个蛋白质组微阵列的技术已经很成熟，到目前为止， 包含来自20种不同传染源的16，000种蛋白质的阵列。通过阵列鉴定的抗原可以是 纯化并翻译到其他可访问的血清学诊断平台，例如96页底部的点阵列， 孔板（多重ELISA）或用于亚单位疫苗的开发。也核心提供探测和 扫描、蛋白质纯化、制备免疫条/多重ELISA（96孔中的宏阵列 板），并通过UCI基因组学和生物信息学研究所进行全面的统计分析。核心 旨在通过开发低成本的基于DNA的基因分型和高通量T 细胞抗原发现 为了支持PSWRCE的活动，核心将实现以下目标 目标i：为新项目提供微阵列服务和可交付成果： a. Posadasii球孢子菌（与亚利桑那大学的John Galgiani合作;项目6.2） B.汉赛巴尔通体（与简克勒，加州大学旧金山分校） 目标2：扩展现有平台。 a.开发高通量T细胞抗原发现（波氏球孢子菌;项目6.2）。 B.开发低成本的基于DNA的基因分型测试（登革热）。 目标3：开发虫媒病毒的多重诊断试验（与Carl Hanson，免疫测定开发 服务核心和Paul Luciw动物资源和实验室服务核心）。 a.免疫试纸条/侧流装置 B.多重ELISA（96孔板中的宏阵列）

The goal of the Protein Micro-Array Core is to provide investigators with a high throughput genome cloning platform from which protein microarrays can be made for discovery of vaccine and serodiagnostic antigens. The technology to make whole proteome microarrays is well established, and to date the Core has fabricated arrays containing 16,000 proteins from 20 different infectious agents. Antigens identified by array can be are purified and translated to other accessible serodiagnostic platforms, such as dot arrays in the bottom of 96 well plates (multiplex ELISA) or for the development of subunit vaccines. The also core offers probing and scanning, protein purification, fabrication of immunostrips /multiplex ELISAs (macroarrays in 96 well plates), and a full statistical analysis through the UCI Institute for Genomice and Bioinformatics. The core aims to expand on these capabilities by developing low cost DNA-baased genotyping and high throughput T cell antigen discovery. To support activities in the PSWRCE, the core will achieve the following aims Aim i: Provide microarray services and deliverables for new projects: a. Coccidioides posadasii (with John Galgiani, University of Arizona; Project 6.2) b. Bartonella henselae (with Jane Koehler, UCSF) Aim 2: Expand on existing platforms. a. Develop high throughput T cell antigen discovery (Coccidioides posadasii; Project 6.2). b. Develop low-cost DNA-based genotyping test (Dengue). Aim 3: Develop multiplex diagnostic tests to Arboviruses (with Carl Hanson, Immunoassay Development Services Core, and Paul Luciw Animal Resources and Laboratory Services Core). a. Immunostrips/Lateral flow devices b. Multiplex ELISA (Macro-arrays in 96-well plates)