ENNCF Proteomics Core

总结 ENNCF蛋白质组学核心的使命是通过尖端的质量分析提供蛋白质分析服务。 质谱法（MS）。我们的主要技术平台是液相色谱-串联质谱 用于高灵敏度蛋白质鉴定、翻译后位点定位和蛋白质分析的LC-MS/MS 量化核心已成为NINDS资助的研究人员的重要技术资源， 埃默里大学是校园内唯一的蛋白质组学核心。在过去的五年里，核心支持了 超过21名NINDS资助的埃默里大学的研究人员代表了不同的部门 包括神经学、细胞生物学、生物化学、药理学、遗传学、生理学和病理学。的 ENNCF蛋白质组学核心的主要目标是：（1）获得最先进的质量 a）LTQ-轨道阱质谱仪，B）四极杆质谱仪， 轨道阱混合质谱仪，c）轨道阱融合三重质谱仪和，d）轨道阱融合 带电子转移解离（ETD）的Tribrid质谱仪，定量蛋白质组学软件和 新兴的肽/蛋白质富集技术，（2）定量蛋白质组学的专业知识和指导 方法、翻译后修改、数据分析和服务，通过与核心 主任和人员，和（3）一个优秀的研究环境，提供基础设施，教育， 和资源，以建立新的和促进目前的合作之间的ENNCF调查人员和其他 丹成功完成蛋白质组学核心实施的目标将刺激 埃默里大学的基础和转化神经科学的多学科研究和创新。 总之，这些目标有一个服务埃默里神经科学界的良好记录， 通过优化实验设计、快速、高效和灵敏的初级检测， 数据收集、正确解释产出和节约成本。这些好处极大地增加了 NINDS通过ENNCF、埃默里医学院和 P30所带来的补贴。

SUMMARY The mission of the ENNCF Proteomics Core is to provide protein analytical services by cutting-edge mass spectrometry (MS). Our main technology platform is liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) for highly sensitive protein identification, post-translational site mapping, and protein quantification. The Core has become an important technological resource for NINDS funded investigators at Emory University and is the only proteomics core on campus. Over the past 5 years the Core has supported over 21 NINDS funded investigators across Emory University representing a diverse number of departments including Neurology, Cell Biology, Biochemistry, Pharmacology, Genetics, Physiology, and Pathology. The primary objectives of the ENNCF Proteomics core are to provide: (1) access to state-of-the-art mass spectrometry that include four mass spectrometers a) LTQ-Orbitrap mass spectrometer, b) Quadrupole- Orbitrap Hybrid Mass Spectrometer, c) Orbitrap Fusion Tribrid Mass Spectrometer and, d) Orbitrap Fusion Tribrid Mass Spectrometer with Electron Transfer Dissociation (ETD), quantitative proteomics software and emerging peptide/protein enrichment technologies, (2) expertise and guidance on quantitative proteomics approaches, post-translational modifications, data analysis and services through direct consultation with core director and personnel, and (3) an outstanding research environment that provides infrastructure, education and resources to both build new and foster current collaborations between ENNCF investigators and other cores. Successful completion of the objectives implemented by the proteomics core will stimulate multidisciplinary research and innovation in basic and translational neuroscience across Emory University. Together these aims have a proven track record of serving the Emory neuroscience community to increase the quality of proteomics data through optimization of experimental design, prompt, efficient and sensitive primary data collection, proper interpretation of output, and cost savings. These benefits accrue overwhelmingly to NINDS funded investigators through support of the Core from ENNCF, the Emory School of Medicine, and subsidies made possible by this P30.