Core Grant for Vision Research - Core #1 Gene Delivery and Quantitative Molecular Biology

核心1：载体和基因传递/定量分子生物学 摘要 核心1的目标是为具有资格拨款和其他愿景的用户提供以下服务 研究人员。这些服务包括就基因传递载体的使用提供咨询、构建质粒 和病毒载体，包装和验证病毒载体，培训个人构建和包装病毒 载体，为定量聚合酶链式反应提供咨询，提供使用定量聚合酶链式反应设备，进行定量聚合酶链式反应 为用户提供反应，提供qPCR数据分析，正常和转基因的等位基因表征 小鼠，核酸探针设计，原位杂交，定量报告基因分析，并便于使用 校园资源，如生物技术中心(NextGen测序、数据处理和分析、 蛋白质组学等)。

CORE 1: VECTOR AND GENE DELIVERY/QUANTITATIVE MOLECULAR BIOLOGY ABSTRACT The goal of Core 1 is to provide the following services to users with qualifying grants and other vision researchers. The services include consultation on the use of gene delivery vectors, construction of plasmid and viral vectors, packaging and validating viral vectors, training individuals to construct and package viral vectors, providing consultation for quantitative PCR, providing access to qPCR equipment, carrying out qPCR reactions for users, providing qPCR data analysis, allelic characterization of normal and genetically modified mice, nucleic acid probe design, in situ hybridization, quantitative reporter gene analysis, and to facilitate use of campus resources such as the Biotechnology Center (NextGen Sequencing, data handling and analysis, proteomics, etc.).