Live Animal Imaging and Functional Analysis Core

摘要 活体动物成像和分析核心 视觉研究需要使用各种动物模型来研究视觉的机制。 发展和退化，致盲性感染和炎症的发病机制，以及 拯救视力的疗法俄克拉荷马州健康大学活体动物成像和分析中心 科学中心负责为视觉研究人员提供最先进的仪器， 在发育、退化、感染和 炎症这些核心支持15个NEI资助的R 01赠款和其他视觉相关的视觉研究 项目在OUHSC在两个不同的地点内vivaria，这允许在/出特权。超过50眼 疾病模型正在用电生理学、功能分析、显微镜和其他方法进行分析。 专门为此目的而更新的扩大研究领域的支助设备。仪表 由该核心提供的方法包括视网膜电图（体内和离体），光学相干断层扫描， 眼底镜检查、验光、激光光凝、眼压测量、间接检眼镜检查和裂隙灯检查， 生物显微成像环境饲养还包括用于光损害、黑暗饲养的设备， 缺氧/高氧、受控湿度和青光眼模型。我们的系统经理在以下方面具有很高的技能 培训人员，协助实验和数据分析，并维护核心设备的质量 标准因此，该核心整合并提供了工具和专业知识， 对于整个校园的视觉研究人员来说，过于昂贵或后勤困难，无法轻松访问。如 上一个资助期，该核心的动机，并致力于提供一个有效的，用户友好的，高， 为所有视觉研究人员提供优质的研究环境。

ABSTRACT Live Animal Imaging and Analysis Core Vision research requires the use of various animal models to investigate the mechanisms of visual development and degeneration, the pathogenesis of blinding infection and inflammation, and the efficacy of vision-saving therapeutics. The Live Animal Imaging and Analysis Core at the University of Oklahoma Health Sciences Center is responsible for providing vision researchers with state-of-the-art instrumentation in the physiology and function of vision in small animal models of development, degeneration, infection, and inflammation. These Cores support vision research on 15 NEI-funded R01 grants and other vision-related projects at OUHSC in two different locations within vivaria, which allows in/out privileges. More than 50 ocular disease models are being analyzed with electrophysiological, functional analysis, microscopy, and other support equipment in expanded research areas renovated specifically for this use. The instrumentation provided by this Core includes electroretinography (in vivo and ex vivo), optical coherence tomography, fundoscopy, optokinetics, laser photocoagulation, tonometry, indirect ophthalmoscopy, and slit lamp and biomicroscopic imaging. Environmental housing also includes equipment for light damage, dark rearing, hypoxia/hyperoxia, controlled humidity, and glaucoma models. Our Systems Managers are highly skilled in training personnel, assisting with experiments and data analysis, and maintaining core equipment to a quality standard. As such, this Core consolidates and provides instrumentation and expertise which would otherwise be too expensive or logistically difficult for vision researchers across campus to easily access. As in the previous funding period, this Core is motivated and committed to providing an effective, user-friendly, and high- quality research environment for all vision researchers at OUHSC.