Single Cell Spatial Analysis in Tissue

项目摘要/摘要 流式细胞术、质量细胞术、细胞分选和成像是一系列方法学的基本工具 旨在了解复杂的单细胞蛋白质组和基因组癌症研究。仪器仪表 所涉及的内容庞大、复杂，并且可能难以维护和操作。因此，有针对性的核心设施至关重要 像MD Anderson这样的综合性癌症中心。FCCIF，Est.1982年，提供这些服务。 自上次CCSG续签(2012)以来，来自所有16个癌症中心项目的404名癌症中心成员使用了 该设施并制作了604份出版物。目前癌症生物学中的许多热门话题都源于免疫。 细胞与肿瘤细胞的相互作用；免疫表型是一个热门的研究领域，需要一个独特的 高丛试验。这需要专门的成像或细胞学方法，而FCCIF特别 收购了两个不同的平台，因为它们的多样性和灵活性。这些平台是Vectra 多光谱成像系统和细胞悬浮与成像(CyTOF)质量细胞仪。FCCIF提供 最先进的专业知识和技术技能的共同来源，以支持MD的各种研究工作 安德森调查人员。FCCIF为所有机构调查人员提供全面的服务 而不考虑以前的专业知识。Jared K.Burks博士(本R50的作者，FCCIF-NC的联席主任)提供 关键服务包括：1)教育，2)咨询所需的技术方法，3)培训 设备使用、分析和适当的实验设计。我确定并获取新技术，以供 MDACC调查人员和我以一种确保成功和减少陷阱的方式提供支持。我在等你 开发新的策略和技术，以满足我们教员的研究需求。以下是几个例子 我目前一直在研究的是Vectra Polaris多光谱显微镜、Helios和Hyperion悬浮液 和成像细胞TOF。在过去的一年里，北极星平均每天运行13.5小时。CyTOF 报告自上次CCSG续订以来增长了5090%。这些结果源于减少陷阱，使 技术平易近人，价格实惠，全程支持研究人员 出版的方式。学术界和产业界的合作导致了小说的开发或测试 申请。我们为支持这些技术而创建的模型已经并将继续共享 与其他人一起运营这些技术。我们高度参与开发和标准化新的 用于MD Anderson的技术以及我们的设施和研究人员在全球实施这些技术。

Project Summary/Abstract Flow Cytometry, Mass Cytometry, Cell Sorting, and Imaging are essential tools for a wide range of methodologies aimed at understanding complex single cell proteomic and genomic cancer research. The instrumentation involved is large, complex, and can be difficult to maintain and operate. As such, a targeted core facility is critical for a Comprehensive Cancer Center such as MD Anderson. The FCCIF, est. 1982, provides these services. Since the last CCSG renewal (2012) 404 cancer center members from all 16 cancer center programs have used the facility and produced 604 publications. Many of the hot topics in cancer biology currently stem from immune cell interaction with the tumor cell; immunephenotyping is an in demand field of research that requiring a unique high plex assay. This requires a specialized imaging or cytometry approach and the FCCIF has specifically acquired two different platforms because of their diversity and flexibility. These platforms are the Vectra Multispectral imaging system and the CyTOF (suspension and imaging) Mass Cytometers. The FCCIF provides a common source of state-of-the-art expertise and technical skills to support the varied research efforts of MD Anderson investigators. The FCCIF provides a comprehensive service to all of the institutional investigators regardless of prior expertise. Dr. Jared K. Burks (author of this R50, co-Director of the FCCIF-NC) provides critical services including: 1) education, 2) consultation on needed technological approach, 3) training on equipment use, analytics, and proper experimental design. I identify and acquire novel technologies for use by MDACC investigators and I provide support in a manner to ensure success and mitigate pitfalls. I am expected to develop new strategies and technologies to meet the research needs of our faculty. A few examples of what I have currently been working on is the Vectra Polaris Multispectral Microscope, Helios and Hyperion Suspension and Imaging CyTOFs. The Polaris has operated on average 13.5 hours per day for the last year. The CyTOFs are reporting 5090% growth since the last CCSG renewal. These results stem from mitigating the pitfalls, making the technologies approachable and affordable, and supporting the researchers throughout the process all the way to publication. Collaborations, both academic and industry, have resulted to develop or test novel applications. The models we have created to support these technologies have and will continue to be shared with others operating the technologies. We are highly involved with both developing and standardizing new technologies for use at MD Anderson and with our facilities and researchers implementing them worldwide.