Molecular Cytogenetics Shared Resource

分子细胞遗传学共享资源：项目总结 分子细胞遗传学共享资源(MCSR)提供对高级DNA修复和基因组的访问 赫伯特·欧文综合癌症中心(HICCC)成员的不稳定性分析。这些服务 包括(1)染色体结构、形态和重排模式的分析(包括 核型、光谱核型(SKY)、染色体涂色和荧光原位杂交[FISH])， (2)特殊基因组区域(端粒、rDNA)的基因组不稳定性分析；(3)DNA损伤反应 和修复途径分析(包括DNA修复蛋白焦点的动力学)，(4)全基因组的发展 分析染色体易位和突变模式的工具(包括高通量序列 平台)，以及(5)咨询和完成实验设计、染色、数据获取和 正在处理。在DNA双链断裂修复专家单查博士的领导下，这些 全面和可定制的服务，以及由MCSR维护的专门工具， 使不同技能水平的研究人员能够描述克隆变化以及非克隆变化的特征 来自广泛生物体的癌细胞和癌前组织中的克隆基因组不稳定性。超过了 目前的项目期(2014-2019年)，MSR已经进行了领导和工具重组。在这 在这一过程中，MCSR购买了新设备并开发了新的检测方法，大大扩展了其能力 定量研究DNA修复机制。重组后的MCSR现在由一位专门的博士管理- 并为HICCC成员提供方便、经济和创新的服务。MCSR 提供不能从商业渠道获得的服务，也不容易在纽约境内获得 并被证明对许多HICCC成员的研究至关重要，提供 赠款申请和影响较大的出版物的主要初步数据。除了提供基本的和 跨DNA修复和基因组不稳定研究的不同领域的高级服务，MCSR还提供 对HICCC成员进行专门仪器的操作以及样品制备和分析方面的培训。 此外，还为调查人员提供咨询服务，以协助设计最具成本效益的 分析并提供验证化验和数据所需的控制。MCSR致力于 结合新的实验组合和分析工具的使用，提供最先进的基因组 稳定服务，并通过培训和咨询促进服务的可获得性，以支持高度 HICCC正在进行协作和创新研究。在本项目期间，MCSR是 供21个HICCC成员使用，支持7份同行审查出版物的关键数据，包括4篇论文 在影响较大的期刊(如《自然》、《分子细胞》)上发表文章，目前支持NIH资助的五项研究 项目，四个来自NCI。

MOLECULAR CYTOGENETICS SHARED RESOURCE: PROJECT SUMMARY The Molecular Cytogenetics Shared Resource (MCSR) provides access to advanced DNA repair and genomic instability analyses to members of the Herbert Irving Comprehensive Cancer Center (HICCC). The services include (1) analyses of chromosomal structural, morphological, and rearrangement patterns (including karyotyping, spectral karyotyping (SKY), chromosome painting, and fluorescence in situ hybridization [FISH]), (2) analyses of genomic instability at specialized genomic regions (telomere, rDNA), (3) DNA damage responses and repair pathway analyses (including the kinetics of DNA repair protein foci), (4) development of genome-wide tools to analyze chromosomal translocation and mutations patterns (including high throughput sequence platforms), and (5) consultation and completed packages for experimental design, staining, data acquisition, and processing. Under the leadership of Shan Zha, PhD, an expert in DNA double-strand breaks repair, these comprehensive and customizable services, together with specialized instruments maintained by the MCSR, enable investigators at varying skill levels with the knowledge to characterize clonal changes as well as non- clonal genomic instability in cancer cells and in premalignant tissues from a wide range of organisms. Over the current project period (2014-2019), MSR has undergone leadership and instrument reorganization. In this process, the MCSR acquired new equipment and developed new assays, which significantly expanded its ability to quantitatively study DNA repair mechanisms. The reorganized MCSR is now managed by a dedicated PhD- level staff and provides convenient, cost-effective, and innovative service to HICCC members. The MCSR provides services that are not available from commercial sources and not easily accessible within the New York metropolitan area and are proven to be critically important to the research of many HICCC members, providing key preliminary data for grant applications and high-impact publications. In addition to providing both basic and advanced services across different area of DNA repair and genomic instability studies, the MCSR also provides training to HICCC members in the operation of specialized instruments and in sample preparation and analyses. In addition, consulting services are available to investigators to assist in the design of the most cost-effective analyses and provide controls needed to validate the assay and the data. The MCSR is committed to incorporating novel experimental portfolios and the use of analytic tools, providing state-of-the-art genomic stability services, and promoting accessibility of services through training and consulting in support of highly collaborative and innovative research ongoing at the HICCC. During the current project period, the MCSR was utilized by 21 HICCC members, supported key data for seven peer-reviewed publications, including four papers in high-impact journals (e.g., Nature, Molecular Cell), and currently supports research for five NIH-funded projects, four from NCI.