Evaluation of zoonotic reservoir hosts for severe fever with thrombocytopenia syndrome virus

项目摘要 严重发热伴血小板减少综合征病毒（SFTSV）是一种新出现的蜱传人类病原体， 中国、日本和韩国。这种病媒，长角血蜱，最近在美国被发现。 因此，国家可以在北美的病毒出现中发挥作用。地方病监测工作 一些地区在家畜和某些野生动物中显示出血清转化和偶尔的病毒RNA，因此表明 人畜共患病传播病毒的可能性。目前，我们对宿主能力和 临床疾病的动物是有限的，这项研究旨在阐明病毒扩增的机制， 脊椎动物宿主的发病机制。这些信息将是了解SFTSV生态的关键， 为监督和监测工作提供指导。我们将保护北美野生动物物种， 亚洲物种的生态相关性，以寻找主管水库主机应SFTSV出现在 美国的此外，我们将开发一个相关的载体宿主模型，用于执行载体能力 研究了H。longicornis使用家养山羊，这是一个可能的病毒来源。这些实验 预计将提供有关疾病传播和传播的感兴趣物种的有力证据， 提供了蜱虫媒介的重要信息

Project Summary Severe fever with thrombocytopenia syndrome virus (SFTSV) is an emerging tick-borne human pathogen in China, Japan and South Korea. The vector, Haemaphysalis longicornis, was recently discovered in the United States, and as such, could play a role in viral emergence in North America. Surveillance efforts in endemic regions show seroconversion and occasional viral RNA in domestic animals and select wildlife, thus indicating the likelihood for zoonotic transmission of the virus. Presently, our knowledge about host competence and clinical disease in animals is limited, and this study seeks to elucidate the mechanism for viral amplification and pathogenesis in vertebrate hosts. This information will be key to understanding the ecology of SFTSV and will provide guidance for surveillance and monitoring efforts. We will inoculate North American wildlife species with ecological relevance to Asian species to look for competent reservoir hosts should SFTSV emerge in the United States. Additionally, we will develop a relevant vector-host model for performing vector competence studies with H. longicornis using domestic goats, which are a likely source of the virus. These experiments are expected to provide strong evidence regarding species of interest in disease transmission and spread and provide vital information about the tick vector.