Design and directed evolution of an 'Edmanase' enzyme for high-throughput peptide sequencing.
Design and directed evolution of an 'Edmanase' enzyme for high-throughput peptide sequencing.
批准号:
10080672
负责人:
KEVIN L GUNDERSON
金额:
$74.97万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
2018
资助国家:
美国
项目状态:
已结题
起止时间:
2018-09-13 至 2022-08-31
关键词:
Amino AcidsApplied ResearchAreaBar CodesBasic ScienceBiological AssayBiological SciencesChemicalsCleaved cellClinical ResearchCouplingDNADNA LibraryDNA sequencingDirected Molecular EvolutionDrug TargetingEngineeringEnzymesGoalsImmobilizationLabelLibrariesMedical ResearchModificationMolecularN-terminalPeptide Sequence DeterminationPeptidesPeriodicityPhaseProcessProtein AnalysisProteinsProteomeReagentSurveysTechnologyVariantbasebiological researchcostcost effectivedesigndigitaldrug developmentgenetic selectionhigh throughput analysisimprovedinstrumentnovelprototypescaffoldscreeningsingle moleculeusabilityuser-friendly
中文摘要
摘要
更易获得和更具成本效益的蛋白质组分析技术将
加快生物和医学研究。这项提议的目标是发展
能够创建高度可扩展的数字蛋白质分析的酶试剂
技术该方法适用范围广,并在成本上有很大改进,
当前方法的敏感度、可用性和吞吐量。
英文摘要
ABSTRACT
More accessible and cost-effective technologies for analysis of the proteome would
accelerate biological and medical research. The goal of this proposal is to develop
enzymatic reagents to enable creation of a highly-scalable digital protein analysis
technology. The approach is broadly applicable and greatly improves upon cost,
sensitivity, usability, and throughput of current approaches.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Development of Reagents for Sequencing Proteins and Protein Fragments on a Next-Generation DNA Sequencer
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批准号:9679623
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项目类别:
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资助金额:$71.83万
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财政年份:2016
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负责人:KEVIN L GUNDERSON
-
依托单位:
Allelic expression monitoring by array-based genotyping
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批准号:6790133
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项目类别:
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资助金额:$19.5万
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财政年份:2004
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负责人:KEVIN L GUNDERSON
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依托单位:
High resolution DNA copy/LOH measurements on WGG arrays
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批准号:6934807
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项目类别:
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资助金额:$58.4万
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财政年份:2003
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负责人:KEVIN L GUNDERSON
-
依托单位:
High resolution DNA copy/LOH measurements on WGG arrays
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批准号:7047919
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项目类别:
-
资助金额:$49.07万
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财政年份:2003
-
负责人:KEVIN L GUNDERSON
-
依托单位:
Representational analysis of DNA copy number/methylation
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批准号:6691586
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项目类别:
-
资助金额:$9.97万
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财政年份:2003
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负责人:KEVIN L GUNDERSON
-
依托单位:
海外基金