Cell Function Analysis Core

华盛顿大学糖尿病研究中心细胞功能分析中心的总体目标 中心为附属机构提供葡萄糖代谢、线粒体功能和细胞内 支持糖尿病、肥胖症和相关疾病的研究。为实现这一目标，核心小组的目标是： (1)使用组织/细胞的体外流动培养系统提供真实的时间功能分析;（2）提供体内 在糖尿病研究中重要的啮齿动物模型中评估代谢表型;（3）提供静态 细胞代谢和功能的评估;（4）从啮齿动物收获、分离和培养原代组织， 包括胰岛和胰岛细胞、肝脏、视网膜和脑，用于随后的形态和功能研究。 表征，以及获取人类胰岛用于相同的目的;（5）提供培训和咨询 （6）开发附属机构要求的新的分析工具，以支持其 研究细胞功能的代谢调节，因为它涉及糖尿病，肥胖症和相关疾病的研究。 紊乱 自2002年核心项目启动以来，体外分析一直是主要关注点。细胞和组织类型， 已经分析的细胞包括胰岛、视网膜、骨骼肌、干细胞、巨噬细胞、淋巴细胞、脂肪细胞， 内皮细胞、神经元细胞和肝/肝细胞。最近，体内服务已被添加到联合收割机 细胞和组织研究中提供的详细和机制分析， 在整个身体环境中识别过程。目前提供的整体动物研究包括葡萄糖和 胰岛素耐量试验、高胰岛素-正常血糖钳夹、胰岛移植和淋巴收集。 新的体外、离体和体内服务的扩展使核心能够更好地服务于 中心的研究基地。由于糖尿病影响许多细胞类型中的代谢和信号传导， 细胞功能分析核心继续对许多中心附属研究人员具有巨大价值。核心计划 继续为用户提供系统和综合的方法来分析细胞类型， 严重参与糖尿病及其并发症、肥胖症和相关疾病。

The overall objective of the Cell Function Analysis Core at the University of Washington Diabetes Research Center is to provide affiliates with analyses of glucose metabolism, mitochondrial function and intracellular signaling to support research of diabetes, obesity and related disorders. To achieve this goal, the Core aims to: (1) Provide real time functional analysis using in vitro flow culture systems of tissues/cells; (2) Provide in vivo assessments of metabolic phenotypes in rodent models important in diabetes research; (3) Provide static assessment of cellular metabolism and function; (4) Harvest, isolate and culture primary tissue from rodents, including islets and islet cells, liver, retina and brain, for subsequent morphological and functional characterization, as well as to procure human islets for the same purposes; (5) Offer training and consultation to affiliates, their trainees and staff; and (6) Develop new analytical tools requested by affiliates to support their studies of the metabolic regulation of cell function as it relates to research in diabetes, obesity and related disorders. Since inception of the Core in 2002, in vitro analysis has been the major focus. Cell and tissue types that have been analyzed include islets, retina, skeletal muscle, stem cells, macrophages, lymphocytes, adipocytes, endothelial cells, neuronal cells and liver/hepatocytes. Recently, in vivo services have been added to combine both the detailed and mechanistic analyses provided in cell and tissue studies with the ability to test the roles of identified processes in whole body settings. Whole animal studies currently offered include glucose and insulin tolerance tests, hyperinsulinemic-euglycemic clamps, islet transplantation and collection of lymph. Expansion of new in vitro, ex vivo, and in vivo services has allowed the Core to better serve the needs of the Center's research base. As diabetes affects metabolism and signaling in many cell types, the services of the Cell Function Analysis Core continue to be of great value to many Center affiliate investigators. The Core plans to continue to provide users with systematic and integrated approaches to the analysis of cell types that are critically involved in diabetes and its complications, obesity and related disorders.