Corneal Biomechanics in Ocular Disease

摘要 这项拟议的研究的目的是调查角膜硬度和角膜硬度的单独影响。 空气脉冲作用下眼内压对角膜变形特性的影响。 将使用成对的人类供体眼睛(n=60双；120只眼)来同时允许眼压和角膜 硬挺度以系统的方式变化，其中一只眼接受角膜治疗 硬化术，对侧眼作为对照，结合4个水平的眼压从 10到40毫米汞柱，以10毫米汞为增量。将使用以下增量更改来修改刚度 丙醛浓度产生两个刚性基团。变形特征将 在使用Corvis ST的~30ms空气脉冲期间拍摄的一系列140张图像中进行测量，A 配备高速摄像头(4,300帧/秒)与水平方向对齐的新设备 使用Scheimpflg几何学的角膜子午线。方差分析将被用来确定 数据的3x4矩阵的差异，以及确定预测因素的逐步回归 基于包括新的刚度的变形参数的IOP和刚度水平 参数。青光眼圆锥角膜变形反应的临床研究(n=50) (n=50)、高眼压(n=50)、糖尿病(n=50)和正常人(n=200) 并对新的刚度参数进行了评估。预期结果包括创建 体外和体内角膜变形反应参数的综合数据库 眼压、角膜硬度参数和活体眼病的函数。

ABSTRACT The purpose of the proposed study is to investigate the separate effects of corneal stiffness and intraocular pressure (IOP) on corneal deformation characteristics under an applied air pulse. Paired human donor eyes (n=60 pairs; 120 eyes) will be used to allow both IOP and corneal stiffness to be varied in a systematic fashion with one eye of the pair treated with a corneal stiffening procedure, and the fellow eye serving as control, combined with 4 levels of IOP from 10 to 40 mmHg in 10mmHg increments. Stiffness will be modified using incremental changes in gluteraldehyde concentration to generate two stiffness groups. Deformation characteristics will be measured in a series of 140 images captured during a ~30ms air pulse with the CorVis ST, a new device with a high speed speed camera (>4,300 frames/sec) aligned with the horizontal meridian of the cornea using Scheimpflug geometry. ANOVA will be used to determine differences in the 3x4 matrix of data, as well as stepwise regression to determine predictors of IOP and level of stiffness based on deformation parameters including a novel stiffness parameter. A clinical investigation of deformation response in keratoconus (n=50), glaucoma (n=50), ocular hypertension (n=50), diabetes (n=50), and normal individuals (n=200) will be conducted with evaluation of novel stiffness parameter. Expected results include the creation of a comprehensive database of ex vivo and in vivo corneal deformation response parameters as a function of IOP, corneal stiffness parameter, and in vivo ocular disease.