The effect of Inhaled Nicotine on Pulmonary Surfaces

摘要 在家长资助中，我们建议研究尼古丁对肺表面的影响。就是我们有 建议研究尼古丁对与肺相关的先天防御关键成分的影响 各种细胞生物学和生物物理技术。其后，我们已要求修订拨款，以 包括人类受试者，这样我们就可以研究尼古丁对源于 包括中性粒细胞在内的人类。然而，在此期间，我们发现了一个重大的知识差距，即 我们并不完全了解吸烟者在吸食过程中和吸食后肺部中的尼古丁含量。这是一个 阻碍了我们的研究，因为我们不知道在体外添加什么浓度的尼古丁 实验。因此，在这份补充材料中，Abdelwahab博士将测量蒸发的人类和小鼠的尼古丁水平 并将在体外进行尼古丁在肺表面的药代动力学研究。这些研究将是 这不仅对我们的研究很重要，而且对该领域的其他研究人员也很重要，因为知识并不存在。 决定如何进行药代动力学将是一个迭代的过程，Abdelwahab博士将 如何以及何时收集尼古丁的智力投入。他还将决定如何使用质量来测量尼古丁 光谱学类型的方法，并将设计实验，以确定尼古丁在 呼吸道表面。这些研究将使Abdelwahab博士能够作为一名科学家发展独立，因为他们将 允许他设计和执行具有国际意义的重要实验。此外，应该 这些实验是成功的，也完全期待他有机会展示这些数据 在国家和国际会议上发表，并撰写在同行评议的期刊上发表。

Abstract In the parent grant, we have proposed to study the effect of nicotine on pulmonary surfaces. That is, we have proposed to look at the effects of nicotine on key components of innate defense relevant to the lung using a variety of cell biological and biophysical techniques. We have subsequently requested a revision to the grant to include human subjects so that we can also study the effects of nicotine on innate defense cells derived from humans including neutrophils. In the interim however, we have identified a major knowledge gap, namely that we do not fully appreciate the levels of nicotine that occur in vapers’ lungs during and after vaping. This is an impediment to our research since we do not know which concentrations of nicotine to add to our in vitro experiments. Thus, in this supplement, Dr. Abdelwahab will measure nicotine levels in vaped humans and mice and will also perform pharmacokinetic studies of nicotine on pulmonary surfaces in vitro. These studies will be important not only for our research but for other researchers in the field since the knowledge does not exist. Deciding how to perform the pharmacokinetics will be an iterative process and Dr. Abdelwahab will have intellectual input into how and when to collect nicotine. He will also decide how to measure nicotine using mass spectrometry-type approaches and will design the experiments to determine how long nicotine stays around on airway surfaces. These studies will enable Dr. Abdelwahab to develop independent as a scientist since they will allow him to design and execute important experiments that will be of international interest. Moreover, should these experiments be successful, it is also fully expected that he will have the opportunity to present these data at national and international meetings and to write them up for publication in peer-reviewed journals.