Crystallography and Computational Biosciences Shared Resource

结晶学与计算生物科学资源共享 项目摘要 结晶学与计算生物科学共享资源(CCBSR)是一个高度专业化的 为维克森林浸信会综合癌症中心(WFBCCC)成员提供访问权限的资源 到专业知识、咨询和最先进的结构和计算生物学方法。由此产生的 数据增强了正在进行的、同行评审的项目和新的资金申请。CCBSR的科学 重要的是需要了解蛋白质-蛋白质、蛋白质-DNA/RNA和 蛋白质-配体/药物相互作用；以及动力学如何影响这些相互作用。CCBSR的使用有 稳步增长，项目的深度和广度也是如此。自2011年−2015年起，联席董事开始工作 与来自11个部门的27个实验室(74%的WFBCCC成员；都有同行评审的资金)一起出版 36篇稿件，解决9个新结构，为18个现有和新资助的赠款进行试验，以及 为新的资金申请收集初步数据。CCBSR有三位联席董事(W.Todd Lowther， 博士；托马斯·霍利斯博士；弗雷德·索尔斯伯里博士)，每个人都有不同的科学、实验和 计算专业知识。因此，在初步协商后，一名或多名联席董事协助 WFBCCC成员与他们的项目开发相匹配，将项目与特定公司的专业知识相匹配- 导演。CCBSR的具体目标是：1)确定适当的结晶学和/或 癌症研究中重要生物分子结构分析的计算方法；2)进行初步 为赠款申请生成初步数据的结晶试验或模拟；以及3)提供设施 并为资助项目提供专业知识，以确定癌症研究中重要生物分子结构。这个 结晶学部分由WFBCCC管理，但也有机构资金。联席董事 参加WFBCCC共享资源季度会议，其中包括关于活动的讨论，最佳 实践、增强的能力和行政管理技术。这些会议提供了一个 概述运营、财务和使用情况，并促进WFBCCC领导层的定期评估。

CRYSTALLOGRAPHY AND COMPUTATIONAL BIOSCIENCES SHARED RESOURCE Project Summary The Crystallography and Computational Biosciences Shared Resource (CCBSR) is a highly-specialized Resource that provides Wake Forest Baptist Comprehensive Cancer Center (WFBCCC) members with access to expertise, consultation, and state-of-the-art structural and computational biology approaches. The resulting data enhance ongoing, peer-reviewed projects and new funding applications. The CCBSR's scientific importance centers on the need to understand the structural basis for protein-protein, protein-DNA/RNA, and protein-ligand/drug interactions; and how dynamics affect these interactions. The use of the CCBSR has steadily increased, as have the depth and breadth of projects. From 2011−2015, the Co-Directors have worked with 27 laboratories from 11 departments (74% WFBCCC members; all with peer-reviewed funding) to publish 36 manuscripts, solve 9 new structures, perform experiments for 18 existing and newly-funded grants, and collect preliminary data for new funding applications. The CCBSR has three co-Directors (W. Todd Lowther, Ph.D.; Thomas Hollis, Ph.D.; Fred Salsbury, Ph.D.), each with different areas of scientific, experimental, and computational expertise. Therefore, after initial consultation, one or more of the Co-Directors assists the WFBCCC member with the development of their project, matching the project to the expertise of a specific Co- Director. The Specific Aims of the CCBSR are to: 1) Determine the appropriate crystallography and/or computational approach for structural analysis of important biomolecules in cancer research; 2) Conduct initial crystallization trials or simulations to generate preliminary data for grant applications; and 3) Provide facilities and expertise for funded projects to determine the structure of important biomolecules in cancer research. The crystallography component is managed by the WFBCCC but also has institutional funding. The Co-Directors attend the quarterly WFBCCC Shared Resources meeting, which includes discussions on activity, best practices, enhanced capabilities, and administrative management techniques. These meetings provide an overview of operations, finances, and utilization, and facilitate regular evaluation by WFBCCC leadership.