Ibis T-6,000 Biosensor System

描述（由申请人提供）：本提案要求为Ibis T6000生物传感器系统提供资金，该系统包含宽范围PCR，然后是电喷雾电离飞行时间（ESI-TOF）质谱分析，以确定PCR扩增子的精确碱基组成。Ibis T6000系统使用专有软件包和广泛的基因组数据库，可以在原始的、复杂的人类或动物标本（包括呼吸道分泌物和粪便）中进行高通量、快速和敏感的微生物（细菌、病毒、真菌）鉴定。该平台可以定制，使其高度适应个人研究项目，它是唯一可用的微生物发现方法，允许潜在的生物体识别到物种水平。最近，该系统被用于诊断美国的第一例2009年猪流感病例。该仪器将直接惠及6个主要用户群体和9个次要用户群体，他们都得到了NIH的研究支持。Ibis T6000将支持的各种研究项目分为3大类：1)确定炎症性肠病、结肠癌和前列腺癌、肝脏疾病进展和有排斥反应和特发性肺部疾病的移植受者的粪便、呼吸或组织微生物组的结构；2)在不同临床环境中检测特定病原体（金黄色葡萄球菌、医院多重耐药革兰氏阴性棒、流感病毒）及其毒力决定因素和抗微生物药物耐药性决定因素；3)在免疫功能低下的宿主中识别危及生命但经常未被诊断的致病病原体，包括弥漫性间质性肺病、腹泻病和败血症。每年约翰霍普金斯大学直接为马里兰州创造了100亿美元的经济活动，比2002年的70亿美元增长了43%相当于今天该州经济中每24美元中的1美元。2008年，约翰霍普金斯大学提供了4.5万个就业岗位，自2002年以来，每年创造700个新就业岗位。约翰霍普金斯大学直接或间接地为马里兰州提供了超过10万个就业岗位，占该州每29个就业岗位的1个。仅在巴尔的摩市，约翰霍普金斯大学就直接或间接地提供了6万个就业岗位，占全市就业岗位的16.7%。该应用程序将立即创造一个工作岗位，并预计通过发现，保留和新的学术和行业/商业职位。此外，此次收购还产生了8个制造和支持Ibis T6000的新职位。

DESCRIPTION (provided by applicant): This proposal requests funds for an Ibis T6000 Biosensor System that incorporates broad-range PCR followed by electrospray ionization time-of-flight (ESI-TOF) mass spectrometric analysis to determine the precise base composition of the PCR amplicons. The Ibis T6000 System then uses a proprietary software package with an extensive genomic database to permit high throughput, rapid and sensitive identification of microbes (bacteria, viruses, fungi) in original, complex human or animal specimens including respiratory secretions and feces. The platform can be customized making it highly adaptable to individual research projects and it is the only microbe discovery method available that permits potential organism identification to the species level. Most recently, this system was used to diagnosis the first swine H1N1 2009 cases in the United States. The instrument will directly benefit 6 major user groups and 9 minor user groups, all of whom have NIH research support. The diverse research projects to be supported by the Ibis T6000 fall into 3 major categories: 1) definition of the structure of the fecal, respiratory or tissue microbiome in inflammatory bowel disease, colon and prostate cancer, progression of liver disease and transplant recipients with rejection and idiopathic lung diseases; 2) detection of specific pathogens (Staphylococcus aureus, nosocomial multidrug resistant gram negative rods, influenza virus), their virulence determinants and antimicrobial resistance determinants in different clinical settings; and 3) identification of disease-causing pathogens in life-threatening, but frequently undiagnosed, illnesses in immunocompromised hosts including diffuse interstitial lung disease, diarrheal disease and septicemia. Every year Johns Hopkins Institutions directly generate about $10 billion in economic activity in the State of Maryland, a 43% increase from the $7 billion generated in 2002 and the equivalent of one of every twenty-four dollars in the state's economy today. In 2008, Johns Hopkins Institutions provided 45,000 jobs and created 700 new jobs each year since 2002. Directly and indirectly Johns Hopkins Institutions support more than 100,000 jobs in Maryland, one of every 29 in the state. In Baltimore City alone Johns Hopkins directly and indirectly supports 60,000 jobs, or 16.7 % of all City employment. This application will create one job immediately and is predicted to yield, through discovery, both retention and new academic and industry/commercial positions. In addition, this acquisition yields eight new positions to manufacture and support the Ibis T6000. PUBLIC HEALTH RELEVANCE: The Ibis T6000 Biosensor System will allow investigations into the contribution of microbes to a wide-range of diseases including respiratory disease in cancer and transplant patients, intestinal diseases such as inflammatory bowel diseases, diarrhea and colon cancer and prostate cancer. The Ibis T6000 Biosensor System will further identify virulent bacteria and viruses such as Staphylococcus aureus, hospital-associated bacteria and influenza that are associated with excess morbidity and mortality. Defining which microbes and how they contribute to these diseases will lead to new approaches to diagnosis, prevention and therapy and make a major contribution to improved public health.