Detection and surveillance of antimicrobial resistance among enteric bacteria from raw retail meat and seafood in Iowa: A NARMS Retail Food Surveillance Project

爱荷华州生零售肉类和海鲜中肠道细菌抗菌药物耐药性的检测和监测：NARMS 零售食品监测项目

• 批准号: 10247550
• 负责人: Orhan Sahin
• 金额: $ 16.7万
• 依托单位: IOWA STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-01 至 2025-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10247550
• 关键词: Detection; surveillance; antimicrobial; resistance; among

Project Summary/Abstract Detection and surveillance of antimicrobial resistance among enteric bacteria from raw retail meat and seafood in Iowa: A NARMS Retail Food Surveillance Project Antimicrobial resistance (AMR) is a major health threat affecting the well-being of humans, animals, and the environment worldwide. Robust and comprehensive surveillance systems emphasizing the “One Health” approach are necessary for effectively combatting AMR. In the United States, the retail arm of the National Antimicrobial Resistance Monitoring System (NARMS) under the Food and Drug Administration, Center for Veterinary Medicine (FDA- CVM) performs surveillance of AMR in select enteric bacteria (non-typhoidal Salmonella, Campylobacter, Enterococcus and Escherichia coli) from retail raw meats (chicken, ground turkey and beef, and ground/chop pork) in 18 states. Recently, surveillance of AMR in retail seafood (tilapia, shrimp, salmon) for select bacteria (Vibrio, Aeromonas, E. coli, Enterococcus, carbapenemase-producing Enterobacteriaciae) has also been started in at least 8 states. The NARMS program has contributed significantly to monitoring the sources and trends of AMR in the food chain. To enhance the effort on combating AMR, FDA launched a cooperative agreement program to expand the NARMS surveillance network throughout the U.S in 2016. Under the initiative, Iowa State University (ISU) became a NARMS network laboratory in late 2016. Since then, the ISU site has contributed substantially to strengthening the capacity and effectiveness of the national AMR surveillance program in retail food. In this application, we propose to continue the collaborative partnership with NARMS to address the updated goals of the Retail Food Surveillance Program as described in FOA PAR-20-124 “NARMS Cooperative Agreement Program to Strengthen Antibiotic Resistance Surveillance in Retail Food Specimens”. Specifically, we will continue to collect and process raw meat samples and seafood samples from grocery stores in Iowa and culture them for isolation of bacteria of interest as defined by NARMS, and ship the isolates to FDA on a monthly basis. In addition, we will continue to perform whole-genome sequencing of Salmonella, Campylobacter and E. coli isolates from the meat samples and share the sequences with FDA via GenomeTrakr. This application is built on existing experience and is a natural extension of our current effort with NARMS. Trained personnel and logistic infrastructure required for successful completion of the planned work are already in place. We strongly believe that ISU’s continued participation in this collaborative network will contribute significantly to mitigation of AMR in the food chain and ultimately to enhancement of food safety and public health.

项目摘要/摘要 RAW的肠细菌中抗菌素耐药性的检测和监测 爱荷华州的零售肉和海鲜：NARMS零售食品监视项目 抗菌耐药性（AMR）是影响福祉的主要健康威胁 人类，动物和全球环境。稳健而全面的监视 强调“一种健康”方法的系统对于有效打击AMR是必要的。 在美国，国家抗菌抗性监测系统的零售部门 （NARMS）在兽医医学中心食品和药物管理局领导下（FDA-- CVM）在某些肠细菌（非类球沙门氏菌， 零售生肉（鸡肉，地面 土耳其和牛肉，以及地面/章色情）在18个州。最近，零售业的AMR监视 选择细菌的海鲜（罗非鱼，虾，鲑鱼） 至少8个州也开始了产生碳青霉烯酶的肠杆菌。这 NARMS计划为监视AMR的来源和趋势做出了重大贡献 食物链。为了加强与AMR战斗的努力，FDA发起了合作社 协议计划在2016年在美国整个美国扩大NARMS监视网络。 在该倡议下，爱荷华州立大学（ISU）最近成为NARMS网络实验室 2016年。从那以后，ISU网站为加强能力和 国家AMR监视计划在零售食品中的有效性。在此应用程序中，我们 提议继续与NARMS的合作伙伴关系，以解决 FOA 20-124中所述的零售食品监视计划“ Narms合作社 协议计划，以加强零售食品中抗生素耐药性监测 标本”。具体来说，我们将继续收集和处理生肉样品和海鲜 来自爱荷华州杂货店的样本，并培养它们，以隔离感兴趣的细菌为 由NARMS定义，并每月将分离株运送到FDA。此外，我们将 继续对沙门氏菌，弯曲杆菌和大肠杆菌进行全基因组测序 从肉样品中分离出来，并通过GenoMetrakr与FDA共享序列。这 应用建立在现有经验上，是我们目前努力的自然延伸 纳姆斯。经过培训的人和逻辑基础设施成功完成了 计划的工作已经到位。我们坚信ISU继续参与此 协作网络将为食物链中的AMR缓解和 最终提高食品安全和公共卫生。