A multiphoton microscope for translational and basic biomedical research

描述（由申请人提供）：本提案寻求资金购买一台奥林巴斯FV1000MPE多光子显微镜，作为威尔康奈尔医学院核心设施的主要仪器。核心设施为转化和基础医学研究人员提供服务。除了威尔康奈尔大学的研究人员外，特殊外科医院的研究人员也将使用该设施。此外，该仪器将作为一个大型合作项目的主要仪器，该项目与伊萨卡康奈尔大学的科学家们正在开发新的方法，包括用于人类患者护理的多光子内窥镜。这个合作项目也是威尔康奈尔大学临床和转化科学中心项目的一个组成部分。该设备的最大用户将是一个由外科医生、病理学家、显微镜学家、工程师和物理学家组成的合作小组，他们正在检查未染色的新鲜人体活检样本，以创建通过自体荧光和二次谐波产生的正常和患病人体组织图谱。初步数据表明，这些图像可以提供与传统组织病理学相当的诊断信息，用于某些类型的癌症。胶原蛋白的SHG也将用于检查骨质疏松症研究的人类骨活检标本。一些研究将在活体动物中进行成像，其中一些项目将受益于基于使用FV1000MPE的协同作用。例如，三位研究人员将研究大脑中的事件，其中两位将合作研究阿尔茨海默病淀粉样斑块的形成和降解。两个项目将检测小鼠体内的巨噬细胞和树突状细胞，并研究细胞迁移等特性。FV1000MPE非常适合满足这组研究人员的需求。它有极好的光学，优化了多光子显微镜和相对有效的成像深入组织。它易于使用，可以快速培训具有不同背景的调查人员。由于其直径小，微探针物镜对动物成像非常有利，并且对于检查不规则形状的手术标本也非常有用，这些手术标本在送去病理处理之前不能被切割或改变。

DESCRIPTION (provided by applicant): This proposal seeks funds to purchase an Olympus FV1000MPE multiphoton microscope to be used as the main instrument in a core facility at the Weill Cornell Medical College. The core facility serves translational and basic medical science investigators. In addition to investigators at Weill Cornell, researchers from the Hospital for Special Surgery will be users of the facility. Additionally, this instrument will serve as the major instrument for a large collaborative project with scientists at Cornell University in Ithaca who are developing novel methods, including multiphoton endoscopy, for use in human patient care. This collaborative project is also a component of the Clinical and Translational Science Center Project based at Weill Cornell. The largest user of the facility will be a collaborative group of surgeons, pathologists, microscopists, engineers, and physicists who are examining unstained fresh human biopsy samples to create an atlas of normal and diseased human tissue as seen by autofluorescence and second harmonic generation. Preliminary data suggest that such images can provide diagnostic information comparable to conventional histopathology for certain types of cancers. SHG from collagen will also be used for examining human bone biopsy specimens for studies of osteoporosis. Several studies will carry out imaging in living animals, and several of these projects will benefit from synergistic interactions based on the use of the FV1000MPE. For example, three investigators will examine events in the brain, and two of these collaborate on studies of the formation and degradation of Alzheimer's disease amyloid plaques. Two projects will examine macrophages and dendritic cells in mice and will investigate properties such as cell migration. The FV1000MPE is very well suited to serve the needs of this group of investigators. It has superb optics that are optimized for multiphoton microscopy and for relatively effective imaging deep into tissue. It is easy to use, which will allow rapid training of investigators with diverse backgrounds. The microprobe objectives are very advantageous for imaging in animals because of their small diameter, and these will also be very useful for examination of irregularly shaped surgical specimens, which cannot be cut or altered before being sent for pathology processing.