ANIMAL EXPERIMENTATION CORE

核心B（动物实验核心）的目标是提高外科手术的可重复性。 程序;病理分析;和功能成像在程序项目的凝聚力的方式。博士 Ravi Ramasamy和Yoshifumi纳卡博士在离体灌注心脏方面具有丰富的经验， 大鼠/小鼠显微手术，并将导致实验诱导I/R应激在老年人与 年轻的小鼠和大鼠在不同的组织。Vivette D 'Agati博士和Matthias Szabolcs博士将协调 进行缺血/再灌注（I/R）应激的小鼠/大鼠组织的病理学分析。测量 损伤;常规免疫组织化学;免疫染色范围/强度的半定量分析 将被执行。Shunichi Homma博士将领导核心的成像功能，以量化 I/R应激触发的梗死程度。核心B将服务于该计划的所有三个项目， 五年计划项目补助金。

The goals of Core B, Animal Experimentation Core, are to enhance the reproducibility of surgical procedures; pathological analysis; and functional imaging in a cohesive manner for the Program Project. Dr. Ravi Ramasamy and Dr. Yoshifumi Naka have extensive experience in the isolated perfused heart and rat/mouse microsurgery, respectively, and will lead the experimentation to induce I/R stress in aged vs. young mice and rats in distinct tissues. Dr. Vivette D'Agati and Dr. Matthias Szabolcs will coordinate pathologic analyses of mouse/rat tissues subjected to ischemia/reperfusion (I/R) stress. Measurement of injury; routine immunohistochemistry; and semi-quantitative analysis of extent/intensity of immunostaining will be performed. Dr. Shunichi Homma will lead the imaging functions of the core, in order to quantify the degree of infarction triggered by I/R stress. Core B will serve all three projects of the Program during each of the five years of the Program Project Grant.