Core 2: Mouse, Cell and Protein Support, Data Management and Biostatistics Core

项目摘要：科学核心 核心2的总体目标是提供关键资源，包括室内饲养的小鼠；来自小鼠的原代细胞 (内皮细胞、心肌细胞和骨髓来源的巨噬细胞)；小鼠的外科手术 三个项目中的两个项目需要。此外，该核心提供RAGE的蛋白/突变蛋白DIAPH1 以及这三个项目所需的Mfn2。此外，整个计划项目由数据提供服务 这一核心的管理和生物统计组成部分。将这些关键活动集中在一起的理由是 确保严格的数据管理和生物统计支持、资源和程序一致性，以及 在整个计划项目中最大限度和最有效地利用资源。所有三个项目都将使用此功能 计划项目的所有五年中的核心2。这一核心的五个具体目标如下：具体目标 1将提供数据管理和生物统计支持；特殊目标2将进行小鼠的繁育 将在本计划项目中使用；特定目标3将执行诱发缺血的外科手术 本计划项目中对小鼠的再灌注损伤；特定目标4将分离和鉴定初级 巨噬细胞、心肌细胞和内皮细胞将用于本计划项目；特定目标5将 RAGE、DIAPH1和Mfn2 Be的纯化蛋白及其定点突变体的制备和鉴定 受雇于本计划项目。目标1-4设在纽约大学医学院/纽约大学朗格尼健康中心， 而AIM 5在奥尔巴尼的纽约州立大学举行。所有必要的设施和设备都可以在 纽约大学医学院/纽约大学朗格尼健康中心和纽约州立大学奥尔巴尼分校成功开展了拟议的 学习。核心领导和核心联合调查员拥有监督所需的所有专业知识和经验 这些核心功能。提供字母并将其附加到本核心，表明不存在重叠 这个核心的五个具体目标是纽约大学或奥尔巴尼大学资助的核心。此核心完全符合 与NHLBI计划项目中的科学核心的说明。核心2服务于 在整个5年的计划项目中提供计划。

Project Summary: Scientific Core The overall goal of Core 2 is to provide the key resources, including mice bred-in-house; primary cells from mice (endothelial cells, cardiomyocytes and bone marrow derived macrophages); and surgical procedures on mice required for two of the three Projects. Further, this Core provides proteins/mutant proteins of RAGE, DIAPH1 and MFN2 that are required for the three Projects. In addition, the entire Program Project is served by the data management and biostatistical component of this Core. The rationale to centralize these key activities is to ensure rigorous data management and biostatistical support, resource and procedural consistency, and maximal and most efficient use of resources throughout the Program Project. All three Projects will use this Core 2 over all five years of the Program Project. The five Specific Aims of this core are as follows: Specific Aim 1 will provide data management and biostatistical support; Specific Aim 2 will perform the breeding of the mice to be employed in this Program project; Specific Aim 3 will perform the surgical procedures to induce ischemia and reperfusion injuries to mice in this Program Project; Specific Aim 4 will isolate and characterize the primary macrophages, cardiomyocytes and endothelial cells to be employed in this Program Project ; Specific Aim 5 will prepare and characterize purified proteins and site-directed mutants of RAGE, DIAPH1 and MFN2 to be employed in this Program Project. AIMS 1-4 are based at the NYU School of Medicine/NYU Langone Health, while AIM 5 takes place at the SUNY at Albany. All of the necessary facilities and equipment are available at NYU School of Medicine/NYU Langone Health and at the SUNY at Albany to successfully carry out the proposed studies. The Core leader and Core Co-Investigator have all of the necessary expertise and experience to oversee these core functions. Letters are provided and appended to this Core indicating that there is NO OVERLAP of the five specific aims of this Core with NYU- or Albany-institutionally funded-Cores. This core is in full compliance with the instructions for scientific cores within NHLBI Program Projects. Core 2 serves all three projects of the Program over all 5 years of the Program Project.