Core 2: Mouse, Cell and Protein Support, Data Management and Biostatistics Core

项目概要：科学核心 Core 2的总体目标是提供关键资源，包括内部培育的小鼠；来自小鼠的原代细胞 （内皮细胞、心肌细胞和骨髓来源的巨噬细胞）；以及对小鼠进行的外科手术 三个项目中的两个需要。此外，该核心提供RAGE、DIAPH1的蛋白质/突变蛋白 和 MFN2 是这三个项目所需的。此外，整个程序项目由数据服务 该核心的管理和生物统计部分。集中这些关键活动的理由是 确保严格的数据管理和生物统计支持、资源和程序的一致性，以及 在整个计划项目中最大限度和最有效地利用资源。所有三个项目都将使用这个 该计划项目全部五年的核心 2。该核心的五个具体目标如下： 具体目标 1. 提供数据管理和生物统计支持；具体目标 2 将进行小鼠的繁殖 受雇于本计划项目；具体目标 3 将进行外科手术以诱发缺血 本项目中小鼠的再灌注损伤；具体目标 4 将隔离并表征初级 本计划项目中使用的巨噬细胞、心肌细胞和内皮细胞；具体目标 5 将 制备并表征纯化的蛋白质以及 RAGE、DIAPH1 和 MFN2 的定点突变体 受雇于该计划项目。 AIMS 1-4 位于纽约大学医学院/纽约大学朗格健康中心， 而 AIM 5 则在纽约州立大学奥尔巴尼分校举行。所有必要的设施和设备都可以在 纽约大学医学院/纽约大学朗格健康中心和纽约州立大学奥尔巴尼分校成功开展了拟议的 研究。核心领导者和核心联合研究员拥有监督所需的所有专业知识和经验 这些核心功能。提供并附加到本核心的信件表明，不存在重叠 该核心与纽约大学或奥尔巴尼机构资助的核心的五个具体目标。该核心完全符合 以及 NHLBI 计划项目中科学核心的说明。 Core 2 服务于所有三个项目 计划涵盖整个计划项目的 5 年。

Project Summary: Scientific Core The overall goal of Core 2 is to provide the key resources, including mice bred-in-house; primary cells from mice (endothelial cells, cardiomyocytes and bone marrow derived macrophages); and surgical procedures on mice required for two of the three Projects. Further, this Core provides proteins/mutant proteins of RAGE, DIAPH1 and MFN2 that are required for the three Projects. In addition, the entire Program Project is served by the data management and biostatistical component of this Core. The rationale to centralize these key activities is to ensure rigorous data management and biostatistical support, resource and procedural consistency, and maximal and most efficient use of resources throughout the Program Project. All three Projects will use this Core 2 over all five years of the Program Project. The five Specific Aims of this core are as follows: Specific Aim 1 will provide data management and biostatistical support; Specific Aim 2 will perform the breeding of the mice to be employed in this Program project; Specific Aim 3 will perform the surgical procedures to induce ischemia and reperfusion injuries to mice in this Program Project; Specific Aim 4 will isolate and characterize the primary macrophages, cardiomyocytes and endothelial cells to be employed in this Program Project ; Specific Aim 5 will prepare and characterize purified proteins and site-directed mutants of RAGE, DIAPH1 and MFN2 to be employed in this Program Project. AIMS 1-4 are based at the NYU School of Medicine/NYU Langone Health, while AIM 5 takes place at the SUNY at Albany. All of the necessary facilities and equipment are available at NYU School of Medicine/NYU Langone Health and at the SUNY at Albany to successfully carry out the proposed studies. The Core leader and Core Co-Investigator have all of the necessary expertise and experience to oversee these core functions. Letters are provided and appended to this Core indicating that there is NO OVERLAP of the five specific aims of this Core with NYU- or Albany-institutionally funded-Cores. This core is in full compliance with the instructions for scientific cores within NHLBI Program Projects. Core 2 serves all three projects of the Program over all 5 years of the Program Project.