Core 2: Mouse, Cell and Protein Support, Data Management and Biostatistics Core

项目概要：科学核心 核心2的总体目标是提供关键资源，包括内部饲养的小鼠;来自小鼠的原代细胞 （内皮细胞、心肌细胞和骨髓衍生的巨噬细胞）;和对小鼠的外科手术 这是三个项目中的两个。此外，该核心提供了DIAPH 1的蛋白质/突变蛋白质 及最惠国待遇2项。此外，整个计划项目由数据提供服务 本核心的管理和生物统计组成部分。集中这些关键活动的理由是， 确保严格的数据管理和生物统计支持、资源和程序的一致性，以及 在整个计划项目中最大限度、最有效地利用资源。这三个项目都将使用 核心2在所有五年的计划项目。该核心的五个具体目标如下：具体目标 1将提供数据管理和生物统计支持; Specific Aim 2将进行小鼠的繁殖 将在本计划项目中使用; Specific Aim 3将执行外科手术以诱导缺血 和再灌注损伤的小鼠在这个程序项目;具体目标4将分离和表征的主要 巨噬细胞、心肌细胞和内皮细胞将在本计划项目中使用;具体目标5将 制备和表征纯化的蛋白质和将要被表达的MFN、DIAPH 1和MFN 2的定点突变体， 在这个计划项目中。AIMS 1-4位于纽约大学医学院/纽约大学Langone Health， 而AIM 5则在纽约州立大学的奥尔巴尼举行。所有必要的设施和设备都可在 纽约大学医学院/纽约大学朗格尼健康和纽约州立大学在奥尔巴尼成功地进行了拟议的 问题研究核心负责人和核心合作研究者拥有所有必要的专业知识和经验来监督 这些核心功能。本核心文件提供并随附了信函，表明没有覆盖 该核心的五个具体目标与纽约大学或奥尔巴尼机构资助的核心。这个核心完全符合 与NHLBI计划项目内的科学核心的说明。核心2服务于所有三个项目的 计划项目的所有5年。

Project Summary: Scientific Core The overall goal of Core 2 is to provide the key resources, including mice bred-in-house; primary cells from mice (endothelial cells, cardiomyocytes and bone marrow derived macrophages); and surgical procedures on mice required for two of the three Projects. Further, this Core provides proteins/mutant proteins of RAGE, DIAPH1 and MFN2 that are required for the three Projects. In addition, the entire Program Project is served by the data management and biostatistical component of this Core. The rationale to centralize these key activities is to ensure rigorous data management and biostatistical support, resource and procedural consistency, and maximal and most efficient use of resources throughout the Program Project. All three Projects will use this Core 2 over all five years of the Program Project. The five Specific Aims of this core are as follows: Specific Aim 1 will provide data management and biostatistical support; Specific Aim 2 will perform the breeding of the mice to be employed in this Program project; Specific Aim 3 will perform the surgical procedures to induce ischemia and reperfusion injuries to mice in this Program Project; Specific Aim 4 will isolate and characterize the primary macrophages, cardiomyocytes and endothelial cells to be employed in this Program Project ; Specific Aim 5 will prepare and characterize purified proteins and site-directed mutants of RAGE, DIAPH1 and MFN2 to be employed in this Program Project. AIMS 1-4 are based at the NYU School of Medicine/NYU Langone Health, while AIM 5 takes place at the SUNY at Albany. All of the necessary facilities and equipment are available at NYU School of Medicine/NYU Langone Health and at the SUNY at Albany to successfully carry out the proposed studies. The Core leader and Core Co-Investigator have all of the necessary expertise and experience to oversee these core functions. Letters are provided and appended to this Core indicating that there is NO OVERLAP of the five specific aims of this Core with NYU- or Albany-institutionally funded-Cores. This core is in full compliance with the instructions for scientific cores within NHLBI Program Projects. Core 2 serves all three projects of the Program over all 5 years of the Program Project.