Tissue Culture/Molecular (TC/M) Core

项目概要（组织培养/分子） 17名视觉科学家（持有13项符合条件的NEI R 01赠款）申请持续资助， 其人员将支持新设立的三个资源/服务核心和一个行政核心， 合并眼科、视觉和解剖科学系（原眼科 和解剖学/细胞生物学部门）。组织培养/分子（TC/M）核心将是 由Jena Steinle博士指导，他是R 01资助的研究员，在组织方面具有重要的专业知识 培养和分子生物学技术。与PI和研究助理一起， 环境和能力进行视觉研究在韦恩州立大学和附属 将通过优先考虑NEI R 01资助的调查人员的工作来加强机构， 他们的工作人员和学生（研究生和研究员）在组织培养和 分子生物学TC/M核心将为视觉科学家（NEI R 01优先资助）提供 确保工作质量和成本效益的设施和技术援助 执行。这个核心将维持和繁殖眼细胞和其他细胞类型，以及提供 研究人员通过基因分型、逆转录-聚合酶链反应（RT）和 定量聚合酶链反应（q）-PCR，基因沉默- siRNA，shRNA，CRISPR/Cas9， 巢式PCR和粘附测定。它还将提供咨询/援助， 重组（r）AAV病毒载体的设计和构建，并协助PI，学生或 进行体外功能评估的工作人员。总的来说，技术合作/管理核心将协助NEI R 01资助的 需要使用组织培养的研究中的研究者及其工作人员/学生， 分子生物学技术;利用组织培养促进视觉科学家之间的合作 和分子生物学技术;并通过以下方式将新的研究人员带入视觉研究 在组织培养和分子技术方面提供帮助， 专心点

Project Summary (Tissue Culture/Molecular) Continuing funds are requested by 17 vision scientists (holding 13 eligible NEI R01 grants) and their personnel to support three resource/service cores and an administrative core in the newly merged Department of Ophthalmology, Visual and Anatomical Sciences (former Ophthalmology and Anatomy/Cell Biology Departments). The Tissue Culture/Molecular (TC/M) core will be directed by Dr. Jena Steinle, an R01-funded investigator with significant expertise in tissue culture and molecular biology techniques. Together with the PI, and a research assistant, the environment and capability to conduct vision research at Wayne State University and affiliated institutions will be enhanced through prioritization of the work of NEI R01 funded investigators, their staff and students (graduate and fellows) in numerous aspects of both tissue culture and molecular biology. The TC/M core will provide vision scientists (priority for NEI R01 funded) with facilities and technical assistance that will assure quality and cost effectiveness of the work performed. This core will maintain and propagate ocular and other cell types, as well as provide investigators with genotyping, reverse transcription-polymerase chain reaction (RT) and quantitative polymerase chain reaction (q)-PCR, gene silencing- siRNA, shRNA, CRISPR/Cas9, nested PCR and adherence assays. It will also provide for consultation/assistance on recombinant (r) AAV virus vector design and construction and assistance to the PI, students or staff for in vitro functional assessments. Overall, the TC/M core will assist NEI R01-funded investigators and their staff/students in studies that require the use of tissue culture and molecular biology techniques; foster collaboration between vision scientists using tissue culture and molecular biological techniques; and bring new investigators into vision research by providing assistance in tissue culture and molecular techniques that can expand their research focus.