Epithelial Cell Reintegration in Development and Disease

项目总结 许多上皮组织是二维片状细胞。来自多家公司的最新工作 系统显示，错位的上皮细胞可以重新整合到板材中，并且 表明这一过程是组织发育的一个共同特征。我们的目标是 了解这一过程。我们之前在果蝇黑腹果蝇上的工作 表明在没有亲同型黏附分子的情况下细胞重新结合失败 线条单元格-单元格边框。这一观察结果提出了一个问题，即同样的 分子在其他系统中调节重新融合。我们的初步工作还表明 这些分子与其他结构成分协调以重塑细胞 在分裂之后，也正是这一进程推动了重新融合。我们提出三个建议 具体目标：1)确定侧向粘连是否在三种情况下调节重新融合 立体培养的脊椎动物组织系统。2a)查明重新融入社会是否 需要黏附分子和细胞骨架组件之间的合作。2B) 确定重组能力是否仅限于新生细胞。这项工作将 阐明细胞重新融合，这是上皮组织的基本属性，并有助于 解释它对组织发育和维护的重要性。

PROJECT SUMMARY Many epithelial tissues are two-dimensional sheets of cells. Recent work from multiple systems shows that a misplaced epithelial cell can reintegrate back into the sheet, and suggest that this process is a common feature of tissue development. We aim to understand this process. Our previous work in the fruit fly Drosophila melanogaster showed that cell reintegration fails in the absence of homophilic adhesion molecules that line cell-cell borders. This observation raises the question of whether the same molecules mediate reintegration in other systems. Our preliminary work also suggests that these molecules coordinate with other structural components to remodel the cell after division, and that it is this process that drives reintegration. We propose three specific aims: 1) Determine whether lateral adhesion mediates reintegration in three- dimensional cultured vertebrate tissue systems. 2A) Find out whether reintegration requires cooperation between adhesion molecules and cytoskeletal components. 2B) Establish whether reintegrative capacity is limited to newly-born cells. This work will illuminate cell reintegration, a fundamental property of epithelial tissues, and help to explain its importance to tissue development and maintenance.